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由来生物
rabbit
品質水準
抗体製品の状態
affinity purified immunoglobulin
抗体製品タイプ
primary antibodies
クローン
polyclonal
化学種の反応性
human, mouse, rat
メーカー／製品名
Chemicon®
テクニック
western blot: suitable
NCBIアクセッション番号
UniProtアクセッション番号
輸送温度
wet ice
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... DFFB(1677)
特異性
Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A mouse DNase that causes DNA fragmentation was identified recently and designated CAD (for caspase activated deoxyribonuclease) (Enari et al. 1998; Sakahira et al. 1998). The human homologue of mouse CAD was more recently identified by three groups independently and termed CPAN and DFF40 and human CAD, respectively (Halenbeck et al. 1998; Liu et al. 1998; Mukae et al. 1998). DFF45/ICAD is the inhibitory protein of DFF40/CAD (Enari et al. 1998; Sakahira et al. 1998; Liu et al. 1997) and forms complex with DFF40/CAD. Upon cleavage of DFF45/ICAD by activated caspase, DFF40/CAD is released and activated and eventually cuases the degradation of DNA in the nuclei. Activation of DFF40/CAD, which causes DNA degradation, is the hallmark of apoptotic cell death.
免疫原
A peptide corresponding to amino acids 147-164 of mouse CAD (Enari et al. 1998). The sequence differs from human DFF40 by two amino acids (Halenbeck et al. 1998; Liu et al. 1998).
アプリケーション
Detect DFF40 using this Anti-DFF40 Antibody validated for use in WB.
Western blot: 1:500-1:1,000
K562 or Jurkat whole cell lysate can be used as a positive control and a 40 kDa band should be detected.
Optimal working dilutions must be determined by end user.
K562 or Jurkat whole cell lysate can be used as a positive control and a 40 kDa band should be detected.
Optimal working dilutions must be determined by end user.
物理的形状
Format: Purified
その他情報
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法的情報
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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保管分類コード
10 - Combustible liquids
WGK
WGK 2
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
AB16926:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Aiying Du et al.
Cell discovery, 1, 15002-15002 (2015-01-01)
In addition to terminating neurotransmission by hydrolyzing acetylcholine, synaptic acetylcholinesterase (AChES) has been found to have a pro-apoptotic role. However, the underlying mechanism has rarely been investigated. Here, we report a nuclear translocation-dependent role for AChES as an apoptotic deoxyribonuclease
Charles W Morgan et al.
Proceedings of the National Academy of Sciences of the United States of America, 112(27), 8344-8349 (2015-06-25)
Cellular demolition during apoptosis is completed by executioner caspases, that selectively cleave more than 1,500 proteins but whose individual roles are challenging to assess. Here, we used an optimized site-specific and inducible protease to examine the role of a classic
María Sánchez-Osuna et al.
The Journal of biological chemistry, 289(27), 18752-18769 (2014-05-20)
Caspase-dependent apoptosis is a controlled type of cell death characterized by oligonucleosomal DNA breakdown and major nuclear morphological alterations. Other kinds of cell death do not share these highly distinctive traits because caspase-activated DNase (DFF40/CAD) remains inactive. Here, we report
B Gole et al.
Oncogene, 34(26), 3391-3401 (2014-08-19)
MLL (myeloid/lymphoid or mixed-lineage leukemia) rearrangements are frequent in therapy-related and childhood acute leukemia, and are associated with poor prognosis. The majority of the rearrangements fall within a 7.3-kb MLL breakpoint cluster region (MLLbcr), particularly in a 0.4-kb hotspot at
Victoria Iglesias-Guimarais et al.
The Journal of biological chemistry, 288(13), 9200-9215 (2013-02-23)
Apoptotic nuclear morphology and oligonucleosomal double-strand DNA fragments (also known as DNA ladder) are considered the hallmarks of apoptotic cell death. From a classic point of view, these two processes occur concomitantly. Once activated, DNA fragmentation factor, 40-kDa subunit (DFF40)/caspase-activated
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