ABE101-S

Anti-monomethyl-Histone H3 (Lys9) Antibody, Trial Size

from rabbit, purified by affinity chromatography

• 別名: H3K9me1, Histone H3 (monomethyl K9), H3 histone family, member T, Histone 3, H3, Histone cluster 3, H3
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: rabbit
• 品質水準: 100
• 抗体製品の状態: purified antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 精製方法: affinity chromatography
• 化学種の反応性: human
• テクニック: dot blot: suitable; immunocytochemistry: suitable; inhibition assay: suitable (peptide); western blot: suitable
• NCBIアクセッション番号: NP_003484.1
• UniProtアクセッション番号: Q16695
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: monomethylation (Lys9)
• 遺伝子情報: human ... HIST1H3F(8968)

詳細

詳細

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

特異性

Broad species cross-reactivity is expected based on sequence homology.

This antibody recognizes Histone H3 methylated at Lys9.

免疫原

Epitope: Lys9

KLH-conjugated branched peptide corresponding to human Histone H3 methylated at Lys9.

アプリケーション

Research Category
エピジェネティクス及び核内機能分子

Research Sub Category
ヒストン

Anti-monomethyl-Histone H3 (Lys9) Antibody, Trial Size is a rabbit polyclonal for detection of Histone H3 monomethylated on lysine 9. Also known as H3K9me1, this antibody has dot blot proven specificity, and has been validated in WB, ICC, PIA. Small pack size is ideal for screening.

Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected Histone H3 in HeLa and A431 cells.

Peptide Inhibition Analysis: 1:10,000 dilution from a representative lot was used with HeLa acid extract for peptide block analysis.

品質

Evaluated by Western Blot in HeLa acid extract lysate.

Western Blot Analysis: 0.1 µg/ml of this antibody detected Histone H3 on 10 µg of HeLa acid extract lysate.

ターゲットの説明

~ 17 kDa

関連事項

Replaces: 07-395

物理的形状

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

アナリシスノート

Control
HeLa acid extract lysate

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable