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由来生物
rabbit
品質水準
抗体製品の状態
serum
抗体製品タイプ
primary antibodies
クローン
polyclonal
化学種の反応性
human, mouse
化学種の反応性（ホモロジーによる予測）
nonhuman primates (based on 100% sequence homology), canine (based on 100% sequence homology), bovine (based on 100% sequence homology), feline (based on 100% sequence homology), horse (based on 100% sequence homology)
テクニック
affinity binding assay: suitable
western blot: suitable
NCBIアクセッション番号
UniProtアクセッション番号
輸送温度
dry ice
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... NR3C1(2908)
詳細
Glucocorticoid receptor (UniProt P04150; also known as GR, Nuclear receptor subfamily 3 group C member 1) is encoded by the NR3C1 (also known as GCCR, GCR, GCRST, GRL) gene (Gene ID 2908) in human. Glucocorticoid receptor is widely expressed in almost all cell types and regulates genes controlling development, metabolism, and immune response upon binding glucocorticoids. Hormone-bound GR translocates from the cytoplasm to the nucleus where it regulates gene transcriptions by binding to glucocorticoid response elements (GREs) or by interacting with various transcription factors. Alternatively, hormone-bound GR can repress gene transcriptions by blocking the nuclear translocation of transcription factors. GR is composed of a modulating domain and a DNA-binding domain at its N-terminal, and a ligand-binding domain at the C-terminal.
特異性
Expected to react with all 10 spliced isoforms reported by UniProt (P04150-1 through P04150-10).
免疫原
Epitope: Modulating region
KLH-conjugated linear peptide corresponding to a sequence from the modulating region of human glucocorticoid receptor.
アプリケーション
Research Category
エピジェネティクス及び核内機能分子
エピジェネティクス及び核内機能分子
Research Sub Category
ホルモン及び受容体
ホルモン及び受容体
Anti-Glucocorticoid Receptor Antibody is an antibody against Glucocorticoid Receptor for use in Western Blotting, Affinity Binding Assay.
Western Blotting Analysis: A representative lot detected glucocorticoid receptor (GR) in mouse liver homogenates (Revollo, J.R., et al. (2013). Sci. Signal. 6(304):ra103).
Western Blotting Analysis: A representative lot detected both wild-type glucocorticoid receptor (GR) and the Dim4 DNA-binding mutant in lysates from U2OS cells that express the respective human GR constructs (Revollo, J.R., et al. (2013). Sci. Signal. 6(304):ra103).
Western Blotting Analysis: A representative lot detected both free glucocorticoid receptor (GR) and dexamethasone mesylate (Dex-Mes) labeled GR in HeLa S3 cell lysates (Cidlowski, J.A., et al. (1990). Mol. Endocrinol 4(10):1427-1437).
Affinity Binding Assay: A representative lot bound monomeric glucocorticoid receptor (GR) as well as oligomeric GR complexes in cytosolic preparations from dexamethasone-/Dex-treated HeLa S3 cells without affecting GR DNA-binding activity (Cidlowski, J.A., et al. (1990). Mol. Endocrinol 4(10):1427-1437).
Western Blotting Analysis: A representative lot detected both wild-type glucocorticoid receptor (GR) and the Dim4 DNA-binding mutant in lysates from U2OS cells that express the respective human GR constructs (Revollo, J.R., et al. (2013). Sci. Signal. 6(304):ra103).
Western Blotting Analysis: A representative lot detected both free glucocorticoid receptor (GR) and dexamethasone mesylate (Dex-Mes) labeled GR in HeLa S3 cell lysates (Cidlowski, J.A., et al. (1990). Mol. Endocrinol 4(10):1427-1437).
Affinity Binding Assay: A representative lot bound monomeric glucocorticoid receptor (GR) as well as oligomeric GR complexes in cytosolic preparations from dexamethasone-/Dex-treated HeLa S3 cells without affecting GR DNA-binding activity (Cidlowski, J.A., et al. (1990). Mol. Endocrinol 4(10):1427-1437).
品質
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: A 1:5,000 dilution of this antibody detected glucocorticoid receptor in 10 µg of HeLa cell lysate.
Western Blotting Analysis: A 1:5,000 dilution of this antibody detected glucocorticoid receptor in 10 µg of HeLa cell lysate.
ターゲットの説明
~95 kDa observed. 85.7 kDa (Alpha; Alpha-A), 81.5 kDa (Beta; Beta-A), 85.8 kDa (Alpha-2; Gamma), 81.7 kDa (Beta-2), 64.8 kDa (GR-A alpha), 60.6 kDa (GR-A beta), 82.8 kDa (Alpha-B). 78.7 kDa (Beta-B) calculated.
物理的形状
Unpurified
Rabbit polyclonal antibody in serum with 0.05% sodium azide.
保管および安定性
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
その他情報
Concentration: Please refer to lot specific datasheet.
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
12 - Non Combustible Liquids
WGK
WGK 1
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
ABE1424:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
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