ABN1445

Anti-PPAR-gamma Antibody

from rabbit, purified by affinity chromatography

• 別名: Peroxisome proliferator-activated receptor gamma, Nuclear receptor subfamily 1 group C member 3, PPAR-gamma, PPARgamma
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• クローン: polyclonal
• application: ICC; WB
• 化学種の反応性: human, mouse
• テクニック: immunocytochemistry: suitable; western blot: suitable
• citations: 4

特性

• 由来生物: rabbit
• 品質水準: 100
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 精製方法: affinity chromatography
• 化学種の反応性: human, mouse
• 化学種の反応性（ホモロジーによる予測）: porcine (based on 100% sequence homology), canine (based on 100% sequence homology), monkey (based on 100% sequence homology), feline (based on 100% sequence homology)
• 包装: antibody small pack of 25 μg
• テクニック: immunocytochemistry: suitable; western blot: suitable
• NCBIアクセッション番号: NP_056953
• UniProtアクセッション番号: P37231
• 輸送温度: ambient
• 保管温度: 2-8°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... PPARG(5468)

詳細

詳細

Peroxisome proliferator-activated receptor gamma (UniProt P37231; also known as Nuclear receptor subfamily 1 group C member 3, PPAR-gamma, PPARgamma) is encoded by the PPARG (also known as GLM1, NR1C3) gene (Gene ID 5468) in human. The peroxisome proliferator-activated receptors (PPARs) are nuclear receptor proteins that function as transcription factors. PPARs target genes play essential roles in the regulation of cellular differentiation, development, metabolism (carbohydrate, lipid, protein), and tumorigenesis. Three types of PPARs exist, namely alpha, delta (beta), and gamma. In addition, alternative splicings produce three PPAR-gamma isoforms. All PPARs heterodimerize with retinoid X receptor (RXR) and bind peroxisome proliferator hormone response elements (PPREs; AGGTCANAGGTCA) in the promoter region of target genes. PPARγ is activated by prostaglandin PGJ2 and certain members of the 5-HETE family of arachidonic acid metabolites, including 5-oxo-15(S)-HETE and 5-oxo-ETE.

特異性

This polyclonal antibody targets an internal sequence present in all spliced isoforms of human and murine PPAR-gamma reported by UniProt (P37231 and P37238).

免疫原

KLH-conjugated linear peptide corresponding to an internal sequence within the N-terminal half of human PPAR-gamma.

アプリケーション

Immunocytochemistry Analysis: 1 µg/mL from a representative lot detected PPAR-gamma in HUVECs, A431, HeLa, HEK293, and NIH/3T3 cells..
Western Blotting Analysis: 1 µg/mL from a representative lot detected PPAR-gamma in 10 µg of human skeletal muscle tissue lysate.

This Anti-PPAR-gamma Antibody is validated for use in Western Blotting, Immunocytochemistry for the detection of PPAR-gamma.

品質

Evaluated by Western Blotting in MCF-7 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected PPAR-gamma in 10 µg of MCF-7 cell lysate.

ターゲットの説明

~55/60 kDa observed. 54.68 kDa (human PPARgamma1(wt); isoform 1), 57.62 kDa (human isoform 2), 21.58 kDa (human PPARgamma1(tr); isoform 3), 54.51 kDa (mouse isoform 1), 57.60 kDa (mouse isoform 2) calculated. Uncharacterized band(s) may appear in some lysates.

関連事項

Replaces: 07-466

その他情報

Concentration: Please refer to lot specific datasheet.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable