ABS229

Anti-HMG-CoA reductase Antibody

from rabbit, purified by affinity chromatography

• 別名: 3-hydroxy-3-methylglutaryl-coenzyme A reductase, HMG-CoA reductase
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• クローン: polyclonal
• application: IP; WB
• 化学種の反応性: human
• テクニック: immunoprecipitation (IP): suitable; western blot: suitable
• citations: 16

特性

• 由来生物: rabbit
• 品質水準: 100
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 精製方法: affinity chromatography
• 化学種の反応性: human
• 化学種の反応性（ホモロジーによる予測）: primate (based on 100% sequence homology)
• テクニック: immunoprecipitation (IP): suitable; western blot: suitable
• NCBIアクセッション番号: NP_000850
• UniProtアクセッション番号: P04035
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... HMGCR(3156)

詳細

詳細

The 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMG-CoA reductase) enzyme is a ubiquitously expressed glycoprotein that is bound to the membrane of the endoplasmic reticulum (ER), but also projects an active C-terminal catalytic tail into the cytoplasm. It is the rate-limiting enzyme in the mevalonate pathway as it catalyzes the conversion of HMG CoA to mevalonate, which is a critical precursor protein in the synthesis of sterols such as cholesterols. In mammalian cells, HMG-CoA reductase is regulated by multiple mechanisms. It is downregulated by high levels of exogenous cholesterol bound to the low density lipoprotein. It is also regulated by sterol and nonsterol metabolites of mevalonate which may exert inhibitory effects at the transcriptional level. Previous studies have suggested that sterols may inhibit the sterol regulatory element-binding proteins (SREBPs) which function as transcription factors that enhance the expression of genes required for sterol biosynthesis. The end-stage degradation process may be mediated by the ubiquitin degradation system. The inhibition of HMG-CoA reductase has been widely studied for the treatment of cholesterol-related conditions.

特異性

Other homologies: Porcine (85% sequence homology).

This antibody recognizes HMG CoA reductase at the linker domain.

免疫原

Epitope: Linker domain

KLH-conjugated linear peptide corresponding to the linker domain of human HMG CoA reductase.

アプリケーション

Anti-HMG-CoA reductase Antibody detects level of HMG-CoA reductase & has been published & validated for use in Immunoprecipitation, Western Blotting.

Immunoprecipitation Analysis: 10 µg/mL from a representative lot immunoprecipitated HMG CoA reductase from HepG2 cell lysate.

品質

Evaluated by Western Blot in HepG2 cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected HMG CoA reductase on 10 µg of HepG2 cell lysate.

ターゲットの説明

~97 kDa observed

関連事項

Replaces: 07-572

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable