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Merck
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主要文書

安全性情報

ABS234

Sigma-Aldrich

Anti-PI3 Kinase Antibody, p85

from rabbit, purified by affinity chromatography

別名:

Phosphatidylinositol 3-kinase regulatory subunit alpha, PI3-kinase regulatory subunit alpha, PI3K regulatory subunit alpha, PtdIns-3-kinase regulatory subunit alpha, Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha, PI3-kinase subunit p85-al

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About This Item

UNSPSCコード:
12352203
eCl@ss:
32160702
NACRES:
NA.41

由来生物

rabbit

品質水準

100

抗体製品の状態

affinity isolated antibody

抗体製品タイプ

primary antibodies

クローン

polyclonal

精製方法

affinity chromatography

化学種の反応性

rat, human

化学種の反応性（ホモロジーによる予測）

mouse (based on 100% sequence homology), monkey (based on 100% sequence homology), bovine (based on 100% sequence homology)

テクニック

immunoprecipitation (IP): suitable
western blot: suitable

NCBIアクセッション番号

NP_037137

UniProtアクセッション番号

Q63787

輸送温度

wet ice

ターゲットの翻訳後修飾

unmodified

遺伝子情報

bovine ... Pik3R1(282307)
human ... PIK3R1(5295)
mouse ... Pik3R1(18708)
rat ... Pik3R1(25513)
rhesus monkey ... Pik3R1(698996)

詳細

Phosphatidylinositol 3-Kinase (PI3 Kinase) is responsible for phosphorylation of the 3 position of the inositol ring of PI(4,5)P2, to generate PI(3,4,5)P3, a potent second messenger required for survival signaling, and insulin action. PI3 Kinase is a heterodimeric complex composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. Tyrosine phosphorylation of growth factor receptors creates docking sites for binding of p85 (through its SH2 domains) on the receptors; p85 brings with it p110, which is then proximal to its phospho-lipid substrate on the membrane. PI3 Kinase is also activated by Ras, and by the β,γ subunits of heterotrimeric G-proteins. PI3 Kinase is inhibited by wortmannin, a useful tool for the study of the PI3 Kinase signaling pathway.

免疫原

GST-tagged recombinant protein corresponding to rat PI3 Kinase, p85.

アプリケーション

Detect PI3 Kinase, p85 using this rabbit polyclonal antibody, Anti-PI3 Kinase Antibody, p85 validated for use in western blotting & IP.
Immunoprecipitation Analysis: A representative lot immunoprecipitated PI3 Kinase, p85 in 0.5 mg of Jurkat cell lysate.

品質

Evaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: A 1:600 dilution of this antibody detected PI3 Kinase, p85 in 10 µg of Jurkat cell lysate.

ターゲットの説明

~85 kDa observed

関連事項

Replaces: 06-497

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保管分類コード

12 - Non Combustible Liquids

WGK

WGK 1

引火点(°F)

Not applicable

引火点(℃)

Not applicable

適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

Jan Code

ABS234:

試験成績書（COA）

製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。

以前この製品を購入いただいたことがある場合

文書ライブラリで、最近購入した製品の文書を検索できます。

文書ライブラリにアクセスする

Bruna Gazzi de Lima-Seolin et al.
Oxidative medicine and cellular longevity, 2019, 6325424-6325424 (2019-07-31)
The increased circulation of norepinephrine, found in the diseased heart as a result of sympathetic nervous system overactivation, is responsible for its cardiotoxic effects including pathological hypertrophy, cell death, and oxidative stress. Bucindolol is a third generation adrenergic blocker, which
Mathews Valuparampil Varghese et al.
American journal of physiology. Lung cellular and molecular physiology, 320(4), L508-L521 (2021-01-28)
We have previously reported that several patients with idiopathic pulmonary hypertension (PH) had different types of G6PD deficiency. However, the role of G6PD in PH is multifactorial because G6PD is involved in controlling oxidative stress, metabolic switch, and red blood
Julie Milanini et al.
Journal of cell science, 131(3) (2017-12-17)
A key step of epithelial morphogenesis is the creation of the lumen. Luminogenesis by hollowing proceeds through the fusion of apical vesicles at cell-cell contacts. The small nascent lumens grow through extension, coalescence and enlargement, coordinated with cell division, to
Flemming Dela et al.
Acta physiologica (Oxford, England), 226(2), e13245-e13245 (2018-12-27)
To examine the effect of high-intensity interval training (HIIT) on glucose clearance rates in skeletal muscle and explore the mechanism within the muscle. Ten males with type 2 diabetes mellitus (T2DM) and ten matched healthy subjects performed 2 weeks of one-legged
Anastasiia Stratiievska et al.
eLife, 7 (2018-12-19)
Although it has been known for over a decade that the inflammatory mediator NGF sensitizes pain-receptor neurons through increased trafficking of TRPV1 channels to the plasma membrane, the mechanism by which this occurs remains mysterious. NGF activates phosphoinositide 3-kinase (PI3K)
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