MAB1596

Anti-DLG4 Antibody

CHEMICON^®, mouse monoclonal, 6G6-1C9

• 別名: PSD-95
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• クローン: 6G6-1C9, monoclonal
• application: ICC; IHC; WB
• 化学種の反応性: mouse, rat, bovine
• テクニック: immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• citations: 151

特性

• 製品名: Anti-Post Synaptic Density Protein 95 Antibody, clone 6G6-1C9, clone 6G6-1C9, Chemicon^®, from mouse
• 由来生物: mouse
• 品質水準: 100
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: 6G6-1C9, monoclonal
• 化学種の反応性: mouse, rat, bovine
• メーカー／製品名: Chemicon^®
• テクニック: immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• アイソタイプ: IgG2a
• NCBIアクセッション番号: NM_001128827.1
• UniProtアクセッション番号: P78352
• 輸送温度: dry ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: bovine ... Dlg4(100137840) ; mouse ... Dlg4(13385) ; rat ... Dlg4(29495)

詳細

詳細

PSD-95 is part of a multiprotein complex restricted to the post-synaptic membrane. It is believed to initiate NMDA receptor clustering on the post synaptic membrane. Its restricted expression makes it an excellent marker for post-synaptic membrane regions on neurons.

特異性

Other species have not been tested.

Reacts with a protein of ~100 kDa, corresponding to the apparent molecular weight of PSD-95, on SDS-PAGE immunoblots of samples from rat, mouse and bovine. A slightly higher molecular weight band is also detected. In rat brain and mouse brain tissue extracts a band of ~75 kDa is also present.

免疫原

Recombinant rat PSD-95.

アプリケーション

Research Category
ニューロサイエンス

Research Sub Category
シナプス及びシナプス生物学

Anti-Post Synaptic Density Protein 95 Antibody, clone 6G6-1C9 detects level of Post Synaptic Density Protein 95 & has been published & validated for use in IC, IH & WB.

Immunocytochemistry:
Representative image from a previous lot.
Confocal fluorescent analysis of PC12 using MAb1596 Mouse mAB (Red). Actin filaments have been labeled with Alexa Fluor 488 -Phalloidin (Green). Nuclear is stained with DAPI (Blue). Membrane staining positive.

Immunocytochemistry:
12.5 µg/mL

Western Blot:
1-5 μg/mL (ECL) on rat brain lysate (20 μg).

Optimal working dilutions must be determined by the end user.

品質

Routinely evaluated in Immunocytochemistry.

ターゲットの説明

~100 kDa

関連事項

Replaces: 04-1066

物理的形状

See datasheet for purification method.

Format: Purified

Purified immunoglobulin. Liquid in PBS, pH 7.2 with 50% glycerol and 0.09% sodium azide.

保管および安定性

Stable for 6 months at -20°C in undiluted aliquots from date of receipt. Do not store below -20°C.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

アナリシスノート

Control
Mouse brain, rat brain tissue.

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法的情報

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 2