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由来生物
mouse
品質水準
抗体製品の状態
purified immunoglobulin
抗体製品タイプ
primary antibodies
クローン
349, monoclonal
化学種の反応性
rat, human, mouse, canine, chicken
メーカー／製品名
Chemicon®
テクニック
ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
アイソタイプ
IgG1
NCBIアクセッション番号
UniProtアクセッション番号
輸送温度
wet ice
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... PXN(5829)
rat ... Pxn(360820)
特異性
Reacts strongly with two bands on Western blots of total cell lysate from chick embryo fibroblasts or rat brain. The lower molecular band is believed to be either an isoform or a degradation product of the 68 kD protein, since it is found also on SDS-PAGE of affinity-purified paxillin.
免疫原
Affinity purified phosphotyrosine-containing proteins from Rous sarcoma virus-transformed chick embryo fibroblasts.
アプリケーション
Research Category
細胞シグナル伝達
細胞シグナル伝達
Research Sub Category
細胞骨格シグナル伝達
細胞骨格シグナル伝達
Anti-Paxillin Antibody, clone 349 detects level of Paxillin & has been published & validated for use in ELISA, WB, IC, IH.
Western blot: 1:1,000-1:10,000
ELISA
Immunohistochemistry 1:900 in Placenta
Immunocytochemistry
Immunoprecipitation
Optimal working dilutions must be determined by end user.
ELISA
Immunohistochemistry 1:900 in Placenta
Immunocytochemistry
Immunoprecipitation
Optimal working dilutions must be determined by end user.
関連事項
Replaces: 04-581
物理的形状
Format: Purified
Purified immunoglobulin. Liquid in aqueous buffered solution containing 0.09% sodium azide.
保管および安定性
Maintain at -20°C in undiluted aliquots for up to 12 months after date of receipt. Avoid repeated freeze/thaw cycles.
During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 μL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container′s cap.
During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 μL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container′s cap.
アナリシスノート
Control
POSITIVE CONTROL:
For western blot, load 5-10 μg of whole cell lysate prepared from the human epidermoid carcinoma cell line, A431.
POSITIVE CONTROL:
For western blot, load 5-10 μg of whole cell lysate prepared from the human epidermoid carcinoma cell line, A431.
その他情報
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法的情報
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
12 - Non Combustible Liquids
WGK
WGK 1
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
MAB3060:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
M Ishino et al.
FEBS letters, 474(2-3), 179-183 (2000-06-06)
Hic-5 is a CAKbeta-binding protein localized at focal adhesions. Here we show that overexpression of CAKbeta or Fyn, but not FAK, enhanced the tyrosine phosphorylation of coexpressed Hic-5 in COS-7 cells. These phosphorylations were further augmented by stimulating cells with
Role of zyxin in differential cell spreading and proliferation of melanoma cells and melanocytes.
van der Gaag, EJ; Leccia, MT; Dekker, SK; Jalbert, NL; Amodeo, DM; Byers, HR
The Journal of Investigative Dermatology null
Imaging morphogenesis, in Xenopus with Quantum Dot nanocrystals.
Stylianou, P; Skourides, PA
Mechanisms of Development null
Mariam Mansour et al.
Journal of cell science, 129(2), 277-289 (2015-12-09)
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ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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