MAB3120

Anti-Cre Recombinase Antibody, clone 2D8

ascites fluid, clone 2D8, Chemicon^®

• 別名: Anti-Cre Antibody
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• クローン: 2D8, monoclonal
• application: ELISA; ICC; IF; IHC; WB
• テクニック: ELISA: suitable; immunocytochemistry: suitable; immunofluorescence: suitable; immunohistochemistry: suitable; western blot: suitable
• citations: 159

特性

• 由来生物: mouse
• 品質水準: 100
• 抗体製品の状態: ascites fluid
• 抗体製品タイプ: primary antibodies
• クローン: 2D8, monoclonal
• 化学種の反応性（ホモロジーによる予測）: all
• メーカー／製品名: Chemicon^®
• テクニック: ELISA: suitable; immunocytochemistry: suitable; immunofluorescence: suitable; immunohistochemistry: suitable; western blot: suitable
• アイソタイプ: IgG1κ
• UniProtアクセッション番号: P06956
• 輸送温度: dry ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: Escherichia coli ... Cre(2777477)

詳細

詳細

Cre-recombinase is a 38 kDa DNA recombinase derived from the P1 bacteriophage. It is highly specific for a 34 bp DNA sequence (loxP) found in P1 DNA. It catalyzes site-specific recombination between two 34-base-pair LOXP sites. Its role is to maintain the phage genome as a monomeric unit-copy plasmid in the lysogenic state. It is homotetramer when bound to DNA and belongs to the "phage" integrase family. Anti-Cre Recombinase Antibody can be used to determine the success of gene manipulations in transgenic animals.

特異性

Cre recombinase. The epitope is located between aa 77 and 343.

免疫原

Cre recombinase (aa 77 to 343) fusion protein

アプリケーション

Research Category
エピジェネティクス及び核内機能分子

Research Sub Category
細胞周期、DNA複製及び修復

Anti-Cre Recombinase Antibody, clone 2D8 is a well characterized monoclonal antibody. Proven to reliably detect Cre Recombinase, this mAb is validated for use in ELISA, IC, IF, IH & WB and is backed by multiple publications.

Western Blot (natural and recombinant protein):
A 1:500-1:5,000 dilution was used in a previous lot. Natural Cre recombinase from Bacteriophage P1 migrates at 38.5 kDa by SDS-PAGE.

Immunofluorescence:
A previous lot of this antibody was used on IF.

Immunohistochemistry:
A 1:500-1:5,000 dilution of a previous lot was used in IH.

ELISA:
A 1:500-1:5,000 dilution of a previous lot was used in ELISA.

Optimal working dilutions must be determined by the end user.

ターゲットの説明

38 kDa

物理的形状

Unpurified

Ascites fluid. Contains no preservative

保管および安定性

Stable for 1 year at -20ºC from date of receipt.

アナリシスノート

Control
HeLa Nuclear Extract

その他情報

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

法的情報

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable