MGAMMAG-300E

MILLIPLEX^® Mouse IgE Single Plex Magnetic Bead Kit - Isotyping Multiplex Assay

Isotyping Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous analysis of multiple immunoglobulin E (IgE) in mouse serum, plasma and cell culture samples.

• UNSPSCコード: 12161503
• eCl@ss: 32161000
• NACRES: NA.47

特性

• 品質水準: 200
• 化学種の反応性: mouse
• メーカー／製品名: Milliplex^®
• assay range: accuracy: 82%; sensitivity: 0.16 ng/mL; standard curve range: 0.8-3500 ng/mL; inter-assay cv: <10%; intra-assay cv: <5%
• テクニック: multiplexing: suitable
• 適合性: configured for Single Plex
• 検出方法: fluorometric (Luminex xMAP)
• 輸送温度: wet ice

詳細

詳細

Found only in mammals, Immunoglobulin E (IgE) plays an essential role in allergic reactions and is predominantly associated with type 1 hypersensitivity. IgE is the least abundant immunoglobulin isotype in normal or topic mammals, present at only 0.05% of IgG serum concentrations. It can trigger robust immune reactions, with up to 10 times the typical level detected in the serum of atopic mammals, although increased levels occur only in approximately 50% of mammals with allergic disorders. IgE binds with high affinity to FcRI, found primarily on mast cells and basophils, priming the cells to recognize specific antigens. Once primed and with repeated exposure to the same antigen, these cells release molecules such as histamines, prostaglandins, and leukotrienes, resulting in an “allergic reaction”.

The MILLIPLEX^® Mouse IgE Single Plex Magnetic Bead Kit is a single plex kit to be used to measure levels of IgE in only 2 μL of mouse urine, serum, plasma or tissue/cell lysate and culture supernatant samples.

The Luminex^® xMAP^® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.

Panel Type: Immune Response

特異性

Cross-reactivity between these antibodies and those against other immunoglobulins is non-detectable or negligible.

アプリケーション

• Analyte: IgE
• Recommended Sample type: Serum, plasma, tissue/cell lysates and tissue culture supernatant
• Recommended Sample dilution: 1:200 dilution for plasma or serum. Tissue/ cell culture supernatants are diluted to approximately 1μg/mL
• Assay Run Time: One day
• Research Category: Immunology and Inflammation, Isotyping

包装

96 Well Plate

保管および安定性

Recommended storage for kit components is 2 - 8°C.

法的情報

Luminex is a registered trademark of Luminex Corp

MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

xMAP is a registered trademark of Luminex Corp

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• ピクトグラム: GHS06,GHS08,GHS05,GHS09
• シグナルワード: Danger
• 危険有害性情報: H302 + H332,H311,H317,H318,H373,H411
• 注意書き: P273 - P280 - P302 + P352 + P312 - P304 + P340 + P312 - P305 + P351 + P338 - P314
• 危険有害性の分類: Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2
• ターゲットの組織: Respiratory Tract
• 保管分類コード: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects