UFC8050

Amicon^® Ultra Centrifugal Filter, 50 kDa MWCO

sample volume 4 mL, regenerated cellulose membrane, MWCO 50 kDa

• 別名: Centrifugal concentrator, 50 kDa MWCO, 4 mL sample volume
• UNSPSCコード: 41104916
• eCl@ss: 36100101
• NACRES: NB.24

特性

• 物質: polyethylene cap; polyethylene liner; polypropylene tube; regenerated cellulose membrane; regenerated cellulose support; styrene-butadiene housing
• 品質水準: 400
• 無菌性: non-sterile
• 製品種目: Amicon^®
• メーカー／製品名: Amicon^® Ultra
• パラメーター: 4 mL sample volume
• テクニック: protein extraction: suitable; protein purification: suitable
• L: 124 mm (4.88 in.)
• 直径: 17.3 mm
• ろ過面積: 3 cm²
• 容量: 4 mL
• ポアサイズ: 50 kDa MWCO
• 輸送温度: ambient

詳細

詳細

Amicon^® Ultra 4 mL centrifugal devices with Ultracel^® 50,000 Dalton molecular weight cutoff filters provide fast ultrafiltration, and reliable retention of macromolecules. For example,BSA (67 kDa) at 1 mg/ml initial concentration is retained at >95% .

The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 80-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss.

The concentrate is collected from the filter device sample reservoir using a pipetter, while the ultrafiltrate is collected in the provided centrifuge tube. The device can be spun in a swinging bucket or fixed angle rotor. With their vertical membrane design and deadstop, Amicon^® Ultra 15 mL filters deliver the highest recovery with short spin times.

アプリケーション

• Ultrafiltration with 50,000 Dalton molecular weight cutoff Ultracel^® membrane.
• Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms.
• Purification of macromolecular components found in tissue culture extracts or cell lysates and protein removal prior to HPLC.
• Concentration of dilute or pre-purified proteins from column eluents.
• Desalting, buffer exchange and protein dialysis.
• Size exclusion and protein fractionation.

特徴および利点

Minimum Final Concentrate Volume: 50 µL

物理的形状

Color Code: 透明　ピンク色キャップ付

法的情報

Amicon is a registered trademark of Merck KGaA, Darmstadt, Germany

ULTRACEL is a registered trademark of Merck KGaA, Darmstadt, Germany