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安全性情報

SFRVN

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Sf-RVN™ Insect Cell Line

別名:

Sf9 rhabdovirus-negative (Sf-RVN™) Insect Cell Line

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About This Item

詳細

Sf9 rhabdovirus-negative (Sf-RVN™) Insect Cell Line

溶解性

DMSO: 10%

輸送温度

liquid nitrogen

保管温度

−196°C

詳細

Sf-RVN(R)cells are provided to customers in vials containing 1 mL at 10 x 106 cells/mL. Cells are banked in EX-CELL® CD Insect Cell Medium and 10% DMSO.
The Sf-RVN™ Insect Cell Line is a rhabdovirus-negative Sf9 cell line that can be grown in both adherent and suspension culture. Sf-RVN™ Insect Cell Line has been adapted for growth in chemically defined medium to be used for the production of recombinant proteins, adeno-associated viruses (AAV) and viral-like particles (VLP). The cell line was tested for adventitious agents and banked under GMP conditions. A technical user guide, with detailed protocols for optimal performances, is provided with the cell line.The EX-CELL® CD Insect Cell Medium, recommended for use, is optimized to get excellent growth and productivity of the Sf-RVN™ Insect Cell Line. Combined, these two products form the Sf-RVN™ Platform.

Cell Line Origin

Sf-RVN™ is an insect cell line originally derived from ovarian tissue of Spodoptera frugiperda (Sf) pupae. The parental line, IPLB-SF-21 (renamed IPLB-SF-21 AE after adaptation into TC-100 medium), was established by J. Vaughn at the Insect Pathology Beltsville in Maryland, USA in the late 1960s. A subclone, designated Sf9, was established in 1983 by G. Smith and C. Cherry. Sf-RVN™ was isolated from Sf9 by GlycoBac, LLC.

物理的形状

Sf-RVN™ cells are provided to customers in vials containing 1 mL at 10 x 106cells/mL. Cells are banked in EX-CELL® CD Insect Cell Medium and 10% DMSO

その他情報

Sf-RVN™ is an insect cell line originally derived from ovarian tissue of Spodoptera frugiperda (Sf) pupae. The parental line, IPLB-SF-21 (renamed IPLB-SF-21 AE after adaptation into TC-100 medium), was established by J. Vaughn at the Insect Pathology Beltsville in Maryland, USA in the late 1960s. A subclone, designated Sf9, was established in 1983 by G. Smith and C. Cherry. Sf-RVN ™ was isolated from Sf9 by GlycoBac, LLC.

法的情報

EX-CELL is a registered trademark of Merck KGaA, Darmstadt, Germany
Sf-RVN is a trademark of Merck KGaA, Darmstadt, Germany

保管分類コード

12 - Non Combustible Liquids

WGK

WGK 1

引火点(°F)

Not applicable

引火点(℃)

Not applicable

適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

毒物及び劇物取締法

劇物
毒物

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名称等を表示すべき危険物及び有害物

労働安全衛生法名称等を通知すべき危険物及び有害物

名称等を通知すべき危険物及び有害物

Jan Code

SFRVN-CDMO-CL-IF:
SFRVN-CDMO-RA-AF:
SFRVN-C2:
SFRVN-C1-AF:
SFRVN-C1-IF:
SFRVN-CDMO-RA-IF:
SFRVN-RA-AF:
SFRVN-C3:
SFRVN-CDMO-CL-AF:
SFRVN-RA-IF:
SFRVN-1VL:4548173371931

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試験成績書（COA）

Lot/Batch Number
2113192341

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資料

Development of a Novel Sf9 Rhabdovirus-Negative Cell Line (Sf-RVN®) and Companion Chemically Defined Medium

See how the Sf9 rhabdovirus-free cell line was developed and how we’ve developed a companion chemically-defined insect cell media for protein and viral expression.

Bioprocessing And Scale-Up Of Suspension HEK293 Cells For AAV Manufacturing

To address scalability challenges of AAV manufacturing, we developed an HEK293 suspension cell line that can be used across many serotypes. Get the data in this article.

Cell and Gene Therapy Manufacturing: Upstream Processing for AAV and Lentivirus Platforms

Minimize the complexities of cell and gene therapy production. Switch to upstream viral vector platforms to enhance scalability and simplify GMP manufacturing.

Can AAV Continue to Deliver the Promise of Gene Therapy?

The current status and future manufacturing of AAV-based gene therapies is discussed in this podcast transcript, including how to streamline large-scale manufacturing.

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関連コンテンツ

Viral Vector Upstream Platforms

A transfection-based solution to viral vector production using a suspension cell line, chemically defined medium, and a process with proven performance at scale.

ウイルスベクターアップストリームプロセス

浮遊培養用細胞株、CD (chemically defined) 培地、およびプロセススケールの性能が実証された、トランスフェクションによるウイルスベクター生産のソリューションです。

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

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