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製品種目
BioReagent
品質水準
フォーム
powder
メーカー／製品名
ATTO-TEC GmbH
λ
in ethanol (with 0.1% trifluoroacetic acid)
UV吸収
λ: 650.0-656.0 nm Amax
適合性
corresponds for gel electrophoresis
suitable for fluorescence
保管温度
−20°C
詳細
Atto 647N is a new generation fluorescent dye dedicated towards the red spectral region. It is a cationic dye and carries a net electrical charge of +1 post substrate coupling reaction. Streptavidin, isolated from Streptomyces avidinii, is a tetrameric protein of 4 x 13.2 kDa which binds very tightly to the small molecule biotin. The dissociation constant of the complex is extremely small (Kd ≈ 10-15 M), ranking among the strongest non-covalent interactions. Atto streptavidin conjugates are readily soluble in water.
アプリケーション
Atto 647N-Streptavidin is designed for application in the area of life science, e.g., labeling of DNA, RNA or proteins. Atto streptavidin conjugates may be used as secondary detection reagents in flow cytometry, immunoassays, blot analysis, and histochemical applications. Atto 647N -Streptavidin is suitable for multiplex analysis, including multicolor imaging.
特徴および利点
Atto 647N- Streptavidin has the following benefits-
- Highly photostable. Strong absorption.
- Excellent fluorescent quantum yield.
- Highly resistant to ozone degradation.
- Minimal triplet formation.
アナリシスノート
dye-to-protein ratio ≥1.5
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保管分類コード
11 - Combustible Solids
WGK
WGK 3
引火点(°F)
Not applicable
引火点(℃)
Not applicable
個人用保護具 (PPE)
Eyeshields, Gloves, type N95 (US)
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
94149-1MG:
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資料
Atto dyes are a series of fluorescent dyes that meet the critical needs of modern fluorescent technologies.
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