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由来生物
mouse
品質水準
結合体
alkaline phosphatase conjugate
抗体製品の状態
purified from hybridoma cell culture
抗体製品タイプ
primary antibodies
クローン
HA-7, monoclonal
形状
buffered aqueous glycerol solution
テクニック
western blot: 1:4,000 using extracts of mammalian cells expressing HA tagged fusion proteins
輸送温度
wet ice
保管温度
2-8°C
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詳細
Monoclonal anti-HA, Alkaline Phosphatase conjugate is derived from the HA-7 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice, immunized with a synthetic peptide. Human influenza hemagglutinin (HA), a surface glycoprotein exists as a trimer. The monomeric HA comprises base and membrane distal-tip regions with conserved residues and receptor-binding site, respectively.
特異性
この抗体は、未変性のみならず変性還元型HA標識タンパク質を認識し、E. coliあるいは哺乳類の細胞で発現されるN-もしくはC-末端HA標識融合タンパク質と反応します。
免疫原
HA標識として知られ、KLHに接合されたヒトインフルエンザウイルス赤血球凝集素（HA）のフラグメント断片に対応する合成ペプチド
アプリケーション
Monoclonal Anti-HA−Alkaline Phosphatase antibody produced in mouse has been used in immunoblotting
生物化学的／生理学的作用
Human influenza hemagglutinin (HA) is required for the infectivity of the human virus. HA interacts with the host cell surface glycoproteins especially the sialic acid to initiate infection. The short sequence derived from the HA molecule corresponding to amino acids 98-106 (YPYDVPDYA) has been used as a tag, known as HA-Tag. This tag facilitates the detection, isolation, purification, coprecipitation of proteins and immunostaining proteins. Caspase 3/7 effectively cleaves HA-tag. Many recombinant proteins or protein of interest have been engineered to express the HA tag fusion protein. The HA-tag does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein.
物理的形状
0.05Mトリスバッファ-溶液(pH 8.0, 1%BSA, 1mM MgCl2, 50%グリセロ-ル, 15mMアジ化ナトリウム(防腐剤)含有)
保管および安定性
For continuous use and extended storage, store at 2-8 °C. Do not freeze. Working dilution samples should be discarded if not used within 12 hours.
免責事項
Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
10 - Combustible liquids
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
A5477-BULK:
A5477-VAR:
A5477-500UG:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Patrick R Arsenault et al.
Molecular and cellular biology, 36(18), 2328-2343 (2016-06-22)
Prolyl hydroxylase domain protein 2 (PHD2) (also known as EGLN1) is a key oxygen sensor in mammals that posttranslationally modifies hypoxia-inducible factor α (HIF-α) and targets it for degradation. In addition to its catalytic domain, PHD2 contains an evolutionarily conserved
Sho Yoshimoto et al.
mAbs, 15(1), 2287250-2287250 (2023-12-04)
PD-1 checkpoint inhibitors have revolutionized the treatment of patients with different cancer histologies including melanoma, renal cell carcinoma, and non-small cell lung carcinoma. However, only a subset of patients show a dramatic clinical response to treatment. Despite intense biomarker discovery
Steven J Gamblin et al.
The Journal of biological chemistry, 285(37), 28403-28409 (2010-06-12)
Considerable progress has been made toward understanding the structural basis of the interaction of the two major surface glycoproteins of influenza A virus with their common ligand/substrate: carbohydrate chains terminating in sialic acid. The specificity of virus attachment to target
The HA tag is cleaved and loses immunoreactivity during apoptosis.
Laura Schembri et al.
Nature methods, 4(2), 107-108 (2007-02-01)
Hansjörg Götzke et al.
Nature communications, 10(1), 4403-4403 (2019-09-29)
Specialized epitope tags are widely used for detecting, manipulating or purifying proteins, but often their versatility is limited. Here, we introduce the ALFA-tag, a rationally designed epitope tag that serves a remarkably broad spectrum of applications in life sciences while
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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