A5968

抗AP-1抗体 ウサギ宿主抗体

affinity isolated antibody, buffered aqueous solution

• 別名: 抗c-Jun抗体
• MDL番号: MFCD02095845
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: rabbit
• 品質水準: 200
• 結合体: unconjugated
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 形状: buffered aqueous solution
• 分子量: antigen 39 kDa
• 化学種の反応性: human
• テクニック: immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200 using formalin-fixed, paraffin-embedded sections of human colon carcinoma; microarray: suitable; western blot: 1:200 using HeLa nuclear extract
• UniProtアクセッション番号: P05412
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... JUN(3725)

詳細

詳細

Activation Protein transcription factor 1 (AP-1) is a leucine zipper transcription factor that recognizes a specific palindromic sequence present in several promoters and enhancers. AP-1 can be induced by several growth and apoptosis-related signaling pathways. Jun proteins are also involved in metastatic, inflammatory and stress responses.
Anti-AP-1/c-Jun recognizes an epitope located on the c-Jun DNA binding domain. This epitope is highly conserved in c-Jun, Jun B and Jun D proteins of chicken, mouse, rat, and human. By immunoblotting, the antibody reacts specifically with AP-1/c-Jun (a single band or occasionally a doublet at 39kDa region). Additional bands of lower molecular weight may be observed. Staining of AP-1/c-Jun band(s) is inhibited by the AP-1/c-Jun immunizing peptide (amino acid residues 246-263).

Activation Protein transcription factor 1 (AP-1) is a regulatory leucine zipper transcription factor. AP-1 is composed of either a homodimer of two Jun protein molecules or a heterodimer of one Jun and one Fos (Proto-oncogenes) molecules.

免疫原

ヒトc-Junのアミノ酸配列246-263に相当する合成ペプチド(N末端リジン付加)のKLH結合体。

アプリケーション

Anti-AP-1 antibody has been used in

• immunohistochemistry
• immunochemistry
• western blotting

Immunofluorescence of Hela cells was performed using monoclonal anti-AP1 (clone 100/3) as the primary antibody.

生物化学的／生理学的作用

Activation Protein transcription factor 1 (AP-1) recognizes a specific sequence (TPA response element, a palindromic TRE sequence TGA(C/G) TCA or related sequences) present in many promoter and enhancer gene regions. Regulation of AP-1 is achieved by changes in the expression of Jun and Fos proteins in the dimer, by phosphorylation at both Ser⁶³ and Ser⁷³ by c-Jun N-terminal kinases (JNK) and by interaction with a variety of transcriptional coactivators like C-Jun activation domain-binding protein-1 (JAB1). AP-1 is induced by a variety of signals including those eliciting proliferation, differentiation and apoptosis.

物理的形状

0.01M PBS溶液 (pH 7.4, 1%BSA, 15mMアジ化ナトリウム含有)。

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable