A7064

抗ビオチン−アルカリフォスファターゼ ヤギ宿主抗体

affinity isolated antibody, buffered aqueous glycerol solution

• 別名: 抗ビオチン
• UNSPSCコード: 12352203
• NACRES: NA.46
• 結合体: alkaline phosphatase conjugate
• application: DB; ELISA (d); IHC (p)
• クローン: polyclonal
• テクニック: direct ELISA: 1:30,000; dot blot: 1:30,000; immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50
• citations: 6

特性

• 由来生物: goat
• 品質水準: 200
• 結合体: alkaline phosphatase conjugate
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• フォーム: buffered aqueous glycerol solution
• テクニック: direct ELISA: 1:30,000; dot blot: 1:30,000; immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50
• 輸送温度: wet ice
• 保管温度: 2-8°C
• ターゲットの翻訳後修飾: unmodified

詳細

詳細

特異性と反応性は、ビオチンキャリアタンパク質複合体に対するオクタロニー二重拡散および免疫電気泳動によって決定します。キャリアタンパク質に対しての反応性は観察されません。

Biotin is a carboxylase co-enzyme that regulates the metabolism of amino acids, glucose and fatty acids. This co-enzyme has been implicated in processes such as immune responses, cell division and fetal growth. Biotin may also be involved in the epigenetic modifications of chromatin.
Goat Anti-Biotin-Alkaline Phosphatase antibody is specific for biotin when tested against biotin-goat IgG, goat IgG, and KLH.

免疫原

ビオチン-KLH結合体

アプリケーション

方法によっては、アビジンとビオチン化プロ-ブの局在化によりバックグラウンド量が高くなることがあります。抗ビオチン試薬をアビジンの代わりに使用すると、非特異的な結合が低減できる場合があります。

ELISA Assays using human spleen cells were performed using alkaline phosphatase-conjugated goat anti-biotin as the secondary antibody with incubation for 1.5 hours at room temperature. Purified IgG from K/BxN, BxSB or C57BL/6 mouse serum was analyzed by western blot using alkaline phosphatase-conjugated goat anti-biotin antibody as the secondary.

Goat Anti-Biotin-Alkaline Phosphatase antibody has been used for dot blot assays. The product can also be used for ELISA (1:30,000) and immunohistochemistry (1:50).

物理的形状

0.05 M Tris溶液(pH 8.0, 10 mM グリシン, 1 mM MgCl₂, 1%BSA, 50%グリセロ-ル, 15 mMアジ化ナトリウム含有)

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable
• 個人用保護具 (PPE): Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)