A7827

Alkaline Phosphatase-Anti-Alkaline Phosphatase antibody produced in mouse

clone AP1B9, purified immunoglobulin, buffered aqueous solution

• 別名: アルカリホスファターゼ標識抗アルカリホスファターゼ抗体(APAAP)
• UNSPSCコード: 12352203
• NACRES: NA.46
• クローン: AP1B9, monoclonal
• application: IHC (p)
• テクニック: immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:40 using human tonsil
• citations: 7

特性

• 由来生物: mouse
• 品質水準: 200
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: AP1B9, monoclonal
• 詳細: Concentrate
• フォーム: buffered aqueous solution
• テクニック: immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:40 using human tonsil
• アイソタイプ: IgG1
• 輸送温度: wet ice
• 保管温度: 2-8°C
• ターゲットの翻訳後修飾: unmodified

詳細

詳細

A soluble complex of calf intestinal alkaline phosphatase and mouse monoclonal antibodies to alkaline phosphatase. The product is a soluble complex of calf intestinal alkaline phosphatase and mouse monoclonal antibodies to alkaline phosphatase.

Monoclonal Anti-Alkaline Phosphatase (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.

アプリケーション

Alkaline Phosphatase-Anti-Alkaline Phosphatase antibody produced in mouse has been used in:

• Immunohistological analysis
• Immunohistochemistry assay
• Immunocytochemical staining

Apoptosis of β-cells in mouse pancreatic sections was analyzed by incubating section with TUNEL and anti-insulin antibody. APAAP was used as the secondary antibody and BCIP/NBT (Sigma) as the substrate.

生物化学的／生理学的作用

Mouse Monoclonal Alkaline Phosphatase Anti-Alkaline Phosphatase is a soluble complex formed from levamisole-resistant calf intestinal alkaline phosphatase and purified mouse monoclonal antibody to alkaline phosphatase. The use of a mouse monoclonal alkaline phosphatase anti-alkaline phosphatase (APAAP) complex, in conjunction with a primary mouse antibody and a secondary bridging antibody (anti-mouse IgG), results in an intense signal with a very low background, while avoiding problems inherent to the covalent conjugation of antibodies. The technology of unlabelled antibody peroxidase-anti peroxidase (PAP) and the alkaline phosphatase anti- alkaline phosphatase (APAAP) method has applications in immunostaining. APAAP monoclonal antibodies complex are homogenous and this method is effective and its sensitive.

物理的形状

Solution in 0.05 M Tris buffered saline, pH 8.0, containing 1 mM MgCl₂, 1% bovine serum albumin and 15 mM sodium azide.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: nwg
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable