A9316

抗マウスIgG (全分子)−アルカリフォスファターゼ ヤギ宿主抗体

affinity isolated antibody, buffered aqueous glycerol solution

• 別名: Goat Anti-Mouse IgG (whole molecule)−AP
• MDL番号: MFCD00162640
• UNSPSCコード: 12352203
• NACRES: NA.46

特性

• 由来生物: goat
• 品質水準: 200
• 結合体: alkaline phosphatase conjugate
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: secondary antibodies
• クローン: polyclonal
• フォーム: buffered aqueous glycerol solution
• テクニック: direct ELISA: 1:30,000; immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50; western blot: 1:30,000
• 輸送温度: wet ice
• 保管温度: 2-8°C
• ターゲットの翻訳後修飾: unmodified

詳細

詳細

Goat polyclonal anti-Mouse IgG (whole molecule)−Alkaline Phosphatase antibody is reactive with mouse IgG1, IgG2a, IgG2b, IgG3, IgA, and IgM. Cross reactivity of the antibody conjugate is determined by ELISA. The conjugate shows no reactivity with rat IgG or rat serum proteins.

Immunoglobulins (Igs) belong to the immunoglobulin super-family. IgG is an abundant immunoglobulin present in human serum. The four classes of IgG are IgG1, IgG2, IgG3, and IgG4.

Mouse IgG is a plasma B cell derived antibody isotype defined by its heavy chain. IgG is the most abundant antibody isotype found in mouse serum. IgG crosses the placental barrier, is a complement activator and binds to the Fc-receptors on phagocytic cells. The level of IgG may vary with the status of disease or infection.

アプリケーション

アルカリホスファターゼ抱合ヤギ抗-マウスIgGを、最終濃度0.5MのNaClを含むTBS/Tween中の濃度μg/mLで2次的に用いて、サーファクタントプロテインAが気管支肺胞液中に検出されました。

Anti-Mouse IgG (whole molecule) Alkaline Phosphatase antibody produced in goat has been used as a secondary antibody in:

• histological studies in heart and liver grafts
• immunohistochemical studies of gangliosides and immunostaining of lymphoid organs
• western blot detection in blood samples

The level of antibodies specific to P. aeuginosa present in immunized mouse sera was determined by ELISA using alkaline phosphatase-conjugated goat anti-mouse IgG as the secondary antibody at a dilution of 1:1000.

生物化学的／生理学的作用

IgG1 deficiency results in hypogammaglobulinemia. IgG2 deficiency increases susceptibility to bacterial infections. IgG3 mediates effector functions and IgG4 is associated with asymptomatic infection.

その他情報

ラット血清タンパクで吸収済みの抗体です。

物理的形状

0.05 M Tris溶液(pH 8.0, 10 mMグリシン, 1% BSA, 1 mM MgCl₂, 50%グリセロ-ル, 15 mMアジ化ナトリウム含有)。

調製ノート

バックグラウンド低減のため、ラットサンプルで吸収済みです。

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable