C4237

Protamine Kinase, Cytosolic from bovine kidney

>90% (SDS-PAGE), buffered aqueous glycerol solution

• 別名: Cytosolic Protamine Kinase
• MDL番号: MFCD04041070
• UNSPSCコード: 12352204
• NACRES: NA.54

特性

• 品質水準: 200
• アッセイ: >90% (SDS-PAGE)
• 形状: buffered aqueous glycerol solution
• 比活性: ≥15,000 units/mg protein
• 分子量: 45 kDa
• 包装: vial of 100 ng
• 輸送温度: dry ice
• 保管温度: −70°C

詳細

アプリケーション

Cytosolic Protamine Kinase (cPK) is isolated from bovine kidney and has a molecular mass of approximately 45 kDa. It phosphorylates the eukaryotic Initiation Factor 4E (eIF4E), which initiates translation by mRNA. It is used to study protein synthesis and various cellular processes.

生物化学的／生理学的作用

Cytosolic protamine kinase is involved in the regulation of protein synthesis and is indirectly associated with numerous cellular processes. Cytosolic protamine kinase is a distinct insulin-stimulated kinase involved in the phosphorylation of eukaryotic Initiation Factor 4E (eIF4E) which is key to initiating translation by mRNA. This protein appears to be inactivated by protein phosphatase 2A family members and may also be inhibited by microcystin, okadeic acid, and ATP. The phosphorylation process is reversible and MBPK1 and MBPK2 (Myelin Basic Protein Kinase 1 and 2) may reactivate cytosolic protamine kinase .

単位の定義

1 unitは、硫酸プロタミンを基質として使用し、pH 7.0、30°C、1分間で、1 nmolのリン酸を取り込みます。

物理的形状

Solution in 50 μl of 50 mM Tris-HCl, pH 7.0, containing 14 mM β-mercaptoethanol, 1mM benzamidine, 0.1 mM PMSF, 1mM EDTA, 50% glycerol

安全性情報

• 保管分類コード: 10 - Combustible liquids