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Merck

C5233

Sigma-Aldrich

カルボキシペプチダーゼB ヒト膵臓由来

50-55 units/mg protein carboxypeptidase B

別名:

ペプチジル-L-リシン(L-アルギニン) ヒドロラーゼ

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About This Item

CAS番号:
Enzyme Commission number:
MDL番号:
UNSPSCコード:
12352204
PubChem Substance ID:
NACRES:
NA.54

由来生物

human pancreas

品質水準

形状

solution

比活性

50-55 units/mg protein carboxypeptidase B

不純物

≤0.2% chymotrypsin
≤0.2% trypsin
≤1 unit/mg protein carboxypeptidase A

UniProtアクセッション番号

輸送温度

dry ice

保管温度

−20°C

InChI

1S/C31H38N4O7S/c1-20(32-26(36)15-16-27(37)38)28(39)33-21(2)30(41)35-17-9-14-25(35)29(40)34-24(18-22-10-5-3-6-11-22)31(42)43-19-23-12-7-4-8-13-23/h3-8,10-13,20-21,24-25H,9,14-19H2,1-2H3,(H,32,36)(H,33,39)(H,34,40)(H,37,38)

InChI Key

TWURVFFNODFJBJ-UHFFFAOYSA-N

遺伝子情報

human ... CPB1(1360)

詳細

Carboxypeptidase B is mapped to human chromosome 3q24. Carboxypeptidase B belongs to A/B subfamily of carboxypeptidases.

アプリケーション

Carboxypeptidase B from Sigma has been used as a reference for assaying carboxypeptidase activity in lysed pituitary granules derived from the anterior and intermediate lobes of rat. The enzyme has also been used to digest plasma samples by removing C-terminal basic amino acids, to get a distinct band for each allotype during C4 electrophoresis.

生物化学的/生理学的作用

Carboxypeptidase B (or peptidyl-L-lysine (-L-arginine) hydrolase) catalyzes the hydrolysis of the basic amino acids, lysine, arginine, and ornithine from the C-terminal position of polypeptides. It has been shown to be a single polypeptide of 34,000 Da. Trypsin is capable of converting native enzyme to the active enzyme, carboxypeptidase B II in vitro. The optimum pH is found to be 9.0. The enzyme may be used for sequence analysis by successive cleavage of C-terminal basic amino acids. It can also be used as a serum marker for the diagnosis of acute pancreatitis.
Mutations in the carboxypeptidase B (CPB1) gene is implicated with increased susceptibility to pancreatic cancer development and progression. Elevated levels of CPB1 is associated with low grade breast tumors and lymph node positive grade 1 tumors.

単位の定義

1 unitは、pH 7.7、25°C、1分間で、1 μmoleのヒップリル-L-アルギニンを加水分解します。

物理的形状

0.05 M NaOAc溶液(1.0 M NaCl, 0.01% NaN3を含有, pH 5.0)

保管分類コード

12 - Non Combustible Liquids

WGK

WGK 1

引火点(°F)

Not applicable

引火点(℃)

Not applicable


適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

Jan Code

C5233-100UG-PW:
C5233-VAR:
C5233-100UG:
C5233-BULK:


試験成績書(COA)

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E Sim et al.
The Biochemical journal, 239(3), 763-767 (1986-11-01)
The plasma complement protein C4 is encoded at two highly polymorphic loci, A and B, within the class-III region of the major histocompatibility complex. At least 34 different polymorphic variants of human C4 have been identified, including non-expressed or 'null'
Xiu Qin Xu et al.
The Journal of biological chemistry, 280(25), 23987-24003 (2005-04-23)
We have exploited a discrepancy in the oncogenic potential of autocrine and exogenous human growth hormone (hGH) in an attempt to identify molecules that could potentially be involved in oncogenic transformation of the human mammary epithelial cell. Microarray analysis of
Saurabh Chatterjee et al.
Free radical biology & medicine, 46(4), 454-461 (2008-12-04)
Post-translational modification of proteins due to exposure to radicals and other reactive species are markers of metabolic and inflammatory oxidative stress such as sepsis. This study uses the nitrone spin-trap DMPO and a combination of immuno-spin trapping and mass spectrometry
Lawrence L K Leung et al.
Advances in experimental medicine and biology, 632, 61-69 (2008-11-26)
Thrombin-activatable procarboxypeptidase B (proCPB or thrombin-activatable fibrinolysis inhibitor or TAFI) is a plasma procarboxypeptidase that is activated by the thrombin-thrombomodulin complex on the vascular endothelial surface. The activated CPB removes the newly exposed carboxyl terminal lysines in the partially digested
J W Brodrick et al.
Biochimica et biophysica acta, 452(2), 468-481 (1976-12-08)
Carboxypeptidase B (peptidyl-L-lysine (-L-arginine) hydrolase, EC 3.4.12.3) has been isolated and purified to apparent homogeneity from activated extracts of human pancreas tissue. The purified enzyme has been shown to be a single polypeptide of 34 000 daltons. In this respect

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