C5301

モノクローナル抗サイトケラチン8ペプチド抗体 マウス宿主抗体

clone M20, ascites fluid

• 別名: Anti-CARD2, Anti-CK-8, Anti-CK8, Anti-CYK8, Anti-K2C8, Anti-K8, Anti-KO
• MDL番号: MFCD00162254
• UNSPSCコード: 12352203
• NACRES: NA.41
• 結合体: unconjugated
• application: IF; IHC (f); WB
• クローン: M20, monoclonal
• 化学種の反応性: feline, human, rabbit, canine, bovine
• citations: 24
• テクニック: immunohistochemistry (frozen sections): suitable; indirect immunofluorescence: 1:200 using frozen sections of human or animal tissue; western blot: suitable

特性

• 由来生物: mouse
• 品質水準: 200
• 結合体: unconjugated
• 抗体製品の状態: ascites fluid
• 抗体製品タイプ: primary antibodies
• クローン: M20, monoclonal
• 分子量: antigen 52 kDa
• 含みます: 15 mM sodium azide
• 化学種の反応性: feline, human, rabbit, canine, bovine
• テクニック: immunohistochemistry (frozen sections): suitable; indirect immunofluorescence: 1:200 using frozen sections of human or animal tissue; western blot: suitable
• アイソタイプ: IgG1
• UniProtアクセッション番号: P05787
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... KRT8(3856)

詳細

詳細

Cytokeratin is a 52.5kD cytoplasmic structural protein that belongs to type II, neutral-to-basic subfamily. It consists of 29 different proteins which is the characteristic of epithelial and trichocytic cells. Monoclonal anti-cytokeratin peptide 8 antibody can be used as a marker for epithelial cell differentiation as well as a tool for tumor identification and classification. It can also be used in western blotting. Mouse anti-cytokeratin peptide 8 antibody reacts specifically with various human simple and complex epithelia like- liver, intestine, pancreas, urinary bladder, salivary gland, thyroid, prostate, mesothel, and placenta but not with stratified squamous epithelia. The product also does not react with non-epithelial tissues (exceptions-certain smooth muscle cells). This product has also shown cross reactivity for cytokeratin of rabbit, cow, dog, and cat.Monoclonal Anti-Cytokeratin 8 (mouse IgG1 isotype) is derived from the M20 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a cytokeratin preparation purified from the human breast carcinoma cell line MCF7.

特異性

Mouse anti-cytokeratin peptide 8 antibody reacts specifically with various human simple and complex epithelia like- liver, intestine, pancreas, urinary bladder, salivary gland, thyroid, prostate, mesothel, and placenta but not with stratified squamous epithelia. The product also does not react with non-epithelial tissues (exceptions-certain smooth muscle cells). This product has also shown cross reactivity for cytokeratin of rabbit, cow, dog, and cat.

免疫原

ヒト乳癌細胞株MCF7由来サイトケラチン。

アプリケーション

Monoclonal anti-cytokeratin peptide 8 antibody has been used in

• immunofluorescence microscopy
• immunohistochemistry
• dot blotting
• protein preparation from bovine epithelial cells
• immunofluorescence
• western blotting

生物化学的／生理学的作用

Monoclonal anti-cytokeratin peptide 8 antibody can be used as a marker for epithelial cell differentiation as well as a tool for tumor identification and classification. It can also be used in western blotting.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable