C7423

モノクローナル抗CD8抗体 マウス宿主抗体

clone UCHT-4, purified immunoglobulin, buffered aqueous solution

• 別名: モノクローナル抗CD8抗体
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 200
• 結合体: unconjugated
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: UCHT-4, monoclonal
• 形状: buffered aqueous solution
• 化学種の反応性: human
• テクニック: flow cytometry: 5 μL using 1 × 10⁶ cells
• アイソタイプ: IgG2a
• UniProtアクセッション番号: P01732; P10966
• 輸送温度: wet ice
• 保管温度: 2-8°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... CD8A(925) , CD8B(926)

詳細

詳細

Monoclonal Anti-Human CD8 (mouse IgG2a isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cell line NS-1 and splenocytes from BALB/c mice immunized with human thymocytes followed by peripheral blood T cells. CD8 (cluster of differentiation 8), is a dimeric protein, encoded by the CD8A gene mapped to human chromosome 2p12.

特異性

ヒトT細胞傷害性/サプレッサ-T細胞表面の糖タンパク質であるCD8を認識します。懸濁状態で胸腺細胞の約90%が染色されます。胸腺切片において、皮質および髄質の胸腺細胞の双方が染色されます。NK細胞サブセットは、CD8を弱く発現します。本抗体が認識するエピト-プは、日常的なホルマリン固定およびパラフィン包埋により損傷されやすいものです。
第3回ワ-クショップ:コ-ド番号567567

免疫原

ヒト胸腺細胞、続いて末梢血 T細胞

アプリケーション

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Flow cytometry/Cell sorting (1 paper)
Immunofluorescence (1 paper)

Monoclonal Anti-CD8 antibody produced in mouse is has been used for the following:

• enumeration of total T cytotoxic/suppressor lymphocytes in bone marrow, blood and other body fluids
• identification and localization of T cytotoxic/ suppressor lymphocytes in lymphoid and other tissues
• analysis of cell mediated cytotoxicity
• studies of immunoregulation in health and disease
• investigation of natural killer (NK) cells
• complement mediated cytolysis of CD8 expressing cells

Monoclonal Anti-CD8 antibody produced in mouse is suitable for flow cytometry at a dilution of 5μL using 1 × 106 cells.

生物化学的／生理学的作用

CD8 might serve as a marker for neoplastic diseases and acute myeloid leukemia.

CD8a (Cluster of Differentiation 8a) is a human gene. CD8 binding to class I MHC and the co-receptor′s during the process of cytotoxic T-cell activation. T cells expresses T cell receptor (TCR) complexes which lacks CD3 delta, exhibit severely impaired positive selection and TCR-mediated activation of CD8 single-positive T cells. CD3 delta couples with TCR. The CD3 associated with CD8 is required for effective activation and positive selection of CD8(+) T cells. CD8 expression is a recurrent rare phenomenon in patients with chronic lymphocytic leukemia (CLL).

ターゲットの説明

CD8は、2本の鎖 (α/α、 α/β) からなる複合体で、Class I MHC分子のレセプタ-です。このレセプタ-の細胞質部位は、周辺のタンパク質をリン酸化するp56^lckタンパク質チロシンキナ-ゼと会合しています。CD8は、成熟T細胞(細胞傷害性/サプレッサ-T細胞)の約3分の1上に強く発現しています。

物理的形状

0.01M PBS溶液(pH 7.4, 1%BSA, 15mMアジ化ナトリウム含有)

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: nwg
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable