C7512

ブチリルコリンエステラーゼ ウマ血清由来

lyophilized powder, ≥10 units/mg protein

• 別名: アシルコリンアシルヒドロラーゼ, コリンエステラーゼ, ブチリル, シュードコリンエステラーゼ
• CAS番号: 9001-08-5
• Enzyme Commission number: 3.1.1.8 (BRENDA, IUBMB)
• EC Number: 232-579-2
• MDL番号: MFCD00081455
• UNSPSCコード: 12352204
• NACRES: NA.54

特性

• 形状: lyophilized powder
• 品質水準: 200
• 比活性: ≥10 units/mg protein
• 分子量: tetramer 440 kDa
• 組成: Protein, ≥60%
• 溶解性: cold water: 60 U/mL
• 保管温度: −20°C

詳細

詳細

Butyrylcholinesterase (BChE) is a serine hydrolase that is structurally similar to acetylcholinesterase (AChE), but differs in substrate specificities and inhibitor sensitivities. The enzyme is a tetrameric glycoprotein with four equal subunits (110 kDa each). It is also termed as pseudocholinesterase. It is produced in the liver. It is found in blood, the synapse of neuromuscular junctions and glia cells and axons of white matter.

アプリケーション

Butyrylcholinesterase from equine serum has been used in a microcalorimetric study of the inhibition of butyrylcholinesterase by paraoxon. This enzyme has also been used in a study to investigate the synthesis and inhibition of cholinergic enzymes. Selective inhibition of BChE activity can be used in the detection of organophosphates. Its use in the treatment of organophosphate toxicity has shown clinical potential, as there is a correlation between the blood level of BChE in humans and the degree of protection against toxic nerve agents. There has also been an interest in the roles of cholinesterases with regard to Alzheimer′s disease. Investigations into selective inhibitors may provide a clearer picture of the physiological role of BChE in both healthy and diseased individuals. This product has been used for the screening of cholineesterase inhibitors in selected fruits and vegetables, for restoring cognitive function and improving memory. It has also been used to develop a butyrylcholinesterase and choline oxidase immobilized bio-sniffer for the detection of nicotine. Nicotine inhibits BChE activity. A decrease in the byproducts of BChE activity reflects the volume of nicotine.

生物化学的／生理学的作用

Butyrylcholinesterase (BChE) can, unlike AChE, efficiently hydrolyze larger esters of choline such as butyrylcholine and benzoylcholine. The enzyme is activated by Ca²⁺ and Mg²⁺ and the activity is constant over the pH range 6.0-8.0. It is inhibited by betaine, nicotine, organophosphates, and carbamates.

Mutations in butyrylcholinesterase (BChE) gene results in the failure of respiratory system, prolonged paralysis and apnea.

単位の定義

1ユニットは、pH 8.0、37°C、1分間に、1.0 μmolのブチリルコリンをコリンと酪酸とに加水分解する酵素量です。ブチリルコリンを基質とした場合の活性は、アセチルコリンを基質とした場合の約2.5倍となります。

物理的形状

バッファ-塩を含有する凍結乾燥品

調製ノート

This product is prepared from equine serum using ammonium sulfate fractionation and supplied as a lyophilized powder.

アナリシスノート

タンパク量はビウレット法で測定

安全性情報

• ピクトグラム: GHS08
• シグナルワード: Danger
• 危険有害性情報: H334
• 注意書き: P261 - P284 - P501
• 危険有害性の分類: Resp. Sens. 1
• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable
• 個人用保護具 (PPE): Eyeshields, Gloves, type N95 (US)