C9281

コレステロールエステラーゼ 蛍光菌Pseudomonas fluorescens由来

lyophilized powder, ≥10,000 units/g protein

• 別名: ステロールエステルアシルヒドロラーゼ
• CAS番号: 9026-00-0
• Enzyme Commission number: 3.1.1.13 (BRENDA, IUBMB)
• EC Number: 232-808-6
• MDL番号: MFCD00071052
• UNSPSCコード: 12352204
• NACRES: NA.54

特性

• 由来生物: Pseudomonas fluorescens
• 品質水準: 200
• アッセイ: 10-30% (TCA-Biuret)
• フォーム: lyophilized powder
• 比活性: ≥10,000 units/g protein
• 分子量: ~129 kDa
• 組成: Protein, ~20%
• 保管条件: under inert gas (argon)
• テクニック: cell based assay: suitable
• 色: tan to brown
• pH: 7-9
• 溶解性: 0.4 M potassium phosphate, pH 7.0: soluble 1.0 mg/mL
• 保管温度: −20°C

詳細

詳細

Research area: Cell Signaling

Cholesterol Esterase (CE) is a glycoprotein that can be isolated from fungal species such as Candida cylindracea and Pseudomonas fluorescens. It is classified as a member of the lipase/esterase family and functions as a homo-dimeric protein. CE is produced in the pancreas and is released in an active form upon stimulation by Cholecystokinin (CCK).

アプリケーション

Cholesterol esterase from Pseudomonas fluorescens has been used in:

• in cholesterol esterase assay to quantify total cholesterol from human blood serum samples
• a study to investigate the nondenaturing protein electro transfer of the esterase activity of lipolytic preparations
• an optimization study of components in enzymatic cholesterol reagents containing cholesterol oxidase
• for the modification of human plasma low-density-lipoprotein (LDL) to induce endothelial cell (EC) dysfunction and monocyte (MC) adhesion in the branched tissue-engineered blood vessels (TEBVs)
• to hydrolyze native cholesterol ester (CE) during ​filipin staining for detection of CE within the retinal frozen sections

This enzyme is widely used in the determination of serum cholesterol in diagnostic laboratories.

生物化学的／生理学的作用

Cholesterol esterase (CE) is a reversible enzyme that can hydrolyze or synthesize fatty acid esters of cholesterol and other sterols. This activity of CE plays a vital role in steroid biosynthesis or de novo steroidogenesis. Hydrolysis of water insoluble long chain fatty acid esters requires bile salt activation. Hydrolysis of water soluble esters of short chain fatty acids and lysophospholipids does not require activation by bile salts. It also hydrolyzes tri-, di-, and mono-acylglycerols, phospholipids, lysophospholipids, and ceramides. The enzyme may have multiple functions in lipid and lipoprotein metabolism, as well as in atherosclerosis..

その他情報

リン酸カリウムとTRITON® X-100を含有します。

単位の定義

1ユニットは、タウロコール酸存在下で、pH 7.0、37°C、1分間で、1.0 μmolのオレイン酸コレステリルをコレステロールとオレイン酸に加水分解する酵素量です。

アナリシスノート

タンパク質はBiuret法で測定。

安全性情報

• ピクトグラム: GHS08
• シグナルワード: Danger
• 危険有害性情報: H334
• 注意書き: P261 - P284 - P501
• 危険有害性の分類: Resp. Sens. 1
• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable
• 個人用保護具 (PPE): Eyeshields, Gloves, type N95 (US)