おすすめの製品
アプリケーション
This enzyme is useful for enzymatic determination of total cholesterol when coupled with cholesterol oxidase in clinical analysis.
生物化学的/生理学的作用
Cholesterol esterase (CE) is a reversible enzyme that can hydrolyze or synthesize fatty acid esters of cholesterol and other sterols. Hydrolysis of water insoluble long chain fatty acid esters requires bile salt activation. Hydrolysis of water soluble esters of short chain fatty acids and lysophospholipids does not require activation by bile salts. It also hydrolyzes tri-, di-, and mono-acylglycerols, phospholipids, lysophospholipids, and ceramide. The enzyme may have multiple functions in lipid and lipoprotein metabolism, and atherosclerosis.
物性
Stability: Stable at –20°C for at least one year
Isoelectric point: 5.9 ± 0.1
Michaelis constants: 5.4 x 10‾5M (Linoleate), 6.6 x 10‾5M (Oleate)
3.7 x 10‾5M (Linolenate), 1.5 x 10‾4M (Palmitate)
1.2 x 10‾4M (Myristate), 2.3 x 10‾5M (Stearate)
Inhibitors: Hg++, Ag+, ionic detergents
Optimum pH: 7.0 − 9.0
Optimum temp: 40°C
pH Stability: pH 5.0 − 9.0 (25°C, 24hr)
Thermal stability: Below 55°C (pH 7.5, 10min)
Isoelectric point: 5.9 ± 0.1
Michaelis constants: 5.4 x 10‾5M (Linoleate), 6.6 x 10‾5M (Oleate)
3.7 x 10‾5M (Linolenate), 1.5 x 10‾4M (Palmitate)
1.2 x 10‾4M (Myristate), 2.3 x 10‾5M (Stearate)
Inhibitors: Hg++, Ag+, ionic detergents
Optimum pH: 7.0 − 9.0
Optimum temp: 40°C
pH Stability: pH 5.0 − 9.0 (25°C, 24hr)
Thermal stability: Below 55°C (pH 7.5, 10min)
単位の定義
1ユニットは、タウロコール酸存在下で、pH 7.0、37°C、1分間で、1.0 μmolのオレイン酸コレステリルをコレステロールとオレイン酸に加水分解する酵素量です。
シグナルワード
Danger
危険有害性情報
危険有害性の分類
Resp. Sens. 1
保管分類コード
11 - Combustible Solids
WGK
WGK 1
引火点(°F)
Not applicable
引火点(℃)
Not applicable
個人用保護具 (PPE)
Eyeshields, Gloves, type N95 (US)
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
C1403-100UN-PW:
C1403-BULK:
C1403-500UN:
C1403-25UN:
C1403-VAR:
C1403-25UN-PW:
C1403-100UN:
C1403-50UN:
C1403-PH:
C1403PROC:
C1403-PPG:
試験成績書(COA)
製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
この製品を見ている人はこちらもチェック
Biomedicines, 10(3) (2022-03-26)
Thioredoxin domain containing 5 (TXNDC5) is a protein disulfide isomerase involved in several diseases related to oxidative stress, energy metabolism and cellular inflammation. In a previous manuscript, a negative association between fatty liver development and hepatic Txndc5 expression was observed.
Journal of lipid research, 43(12), 2017-2030 (2002-11-28)
Carboxyl ester lipase (CEL), previously named cholesterol esterase or bile salt-stimulated (or dependent) lipase, is a lipolytic enzyme capable of hydrolyzing cholesteryl esters, tri-, di-, and mono-acylglycerols, phospholipids, lysophospholipids, and ceramide. The active site catalytic triad of serine-histidine-aspartate is centrally
Journal of lipid research, 53(11), 2296-2306 (2012-09-04)
Lipoic acid (LA) is a naturally occurring compound with beneficial effects on obesity. The aim of this study was to evaluate its effects on lipolysis in 3T3-L1 adipocytes and the mechanisms involved. Our results revealed that LA induced a dose-
American journal of physiology. Endocrinology and metabolism, 303(9), E1126-E1133 (2012-09-06)
High concentrations of TNF within obese adipose tissue increase basal lipolysis and antagonize insulin signaling. Adipocytes of the obese are also exposed to elevated levels of glucocorticoids (GCs), which antagonize TNF actions in many cell types. We tested the hypothesis
Experimental & molecular medicine, 44(10), 603-614 (2012-08-04)
Higher levels of body fat are associated with an increased risk for development numerous adverse health conditions. FTY720 is an immune modulator and a synthetic analogue of sphingosine 1-phosphate (S1P), activated S1P receptors and is effective in experimental models of
資料
Cholesterol esterification enhances transport efficiency in lipoproteins for increased blood stream transport.
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
製品に関するお問い合わせはこちら(テクニカルサービス)