CRISPR17

CRISPR Activator Human Oct4 Positive Control Plasmid

gRNA Only Expression Plasmid

• UNSPSCコード: 41106609
• NACRES: NE.02

特性

• 品質水準: 200
• フォーム: liquid
• 包装: vial of 50 μL
• 濃度: 20 ng/μL in TE buffer; DNA (1μg of plasmid DNA)
• アプリケーション: CRISPR
• 輸送温度: dry ice
• 保管温度: −20°C

詳細

詳細

OCT4 is the key transcription factor (TF) that maintains pluripotency of embryonic stem cells and plays a critical role in reinstating cellular pluripotency. The expression of OCT4 is stringently silenced in differentiated cells. Activation of silenced OCT4 gene has become a hallmark event during epigenetic reprogramming into iPSCs. The positive control OCT4 gRNA design has been tested with the dCas9-p300 plasmid in HEK293 cells and shown to increase OCT4 mRNA transcript levels over three-fold compared to a negative control.

アプリケーション

Functional Genomics/Target Validation

• Epigenetic Modification
• Transcriptional Activation

特徴および利点

Serves as an experimental control for the CRISPR dCas9-p300 activation system. A positive experiment will result in an increased level of mRNA via qPCR or TaqMan PCR assay.

構成

1 vial containing 1ug of U6-gRNA plasmid expressing a guide sequence to human OCT4 (Octamer-binding transcription factor-4 or POU5F1).

関連事項

To order U6-gRNA plasmids, please visit sigma.com/crisprs

Click here for more information on dcas9-p300 Gene Activator Expression Plasmid.

その他情報

Must be used with dCas9-p300 or other dCas9 activator/repressor plasmids.

法的情報

CRISPR Use License Agreement

Lentiviral and WPRE License Agreements

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: nwg
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable