D6944

Anti-Dopamine Transporter (N-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

• 別名: Anti-DAT, Anti-DAT1, Anti-SLC6A3, Anti-Solute carrier family 6, member 3
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: rabbit
• 品質水準: 200
• 結合体: unconjugated
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 形状: buffered aqueous solution
• 分子量: antigen ~50 kDa
• 化学種の反応性: mouse (predicted), rat, human
• 強化検証: recombinant expression; Learn more about Antibody Enhanced Validation
• 濃度: ~1.0 mg/mL
• テクニック: immunofluorescence: 5-10 μg/mL using SH-SY-5Y cells; western blot: 0.5-1 μg/mL using rat brain tissue extract
• UniProtアクセッション番号: Q01959
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... SLC6A3(6531) ; mouse ... Slc6a3(13162) ; rat ... Slc6a3(24898)

詳細

詳細

Dopamine transporter belongs to a family of biogenic amine transporters that include the norepinephrine, serotonin (SERT), and vesicular monoamine transporters. These transporters are members of a larger Na⁺/Cl^--dependent neurotransmitter transporter family.

Solute carrier family 6 member 3 (SLC6A3) is mapped to human chromosome 5p15.33. It is also known as the dopamine transporter (DAT) and has a zinc binding site and 12 transmembrane region.

免疫原

synthetic peptide corresponding to amino acids 42-59 of rat dopamine transporter conjugated to KLH. This sequence is highly conserved in mouse DAT (single amino acid substitution) and has lower homology (72% identity) to human DAT.

アプリケーション

Anti-Dopamine Transporter antibody produced in rabbit is suitable for western blot analysis at a working concentration of 0.5-1μg/mL using rat brain extract (cytosolic S1 fraction) and HEK-293T cells expressing human DAT1. It was used as a primary antibody in western analysis for dopamine transporter in several studies.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunocytochemistry (1 paper)
Immunohistochemistry (1 paper)
Western Blotting (1 paper)

生物化学的／生理学的作用

DAT regulates dopaminergic-neurotransmission. It is localized on presynaptic nerve terminals and mediates the active reuptake of dopamine from the synapse, thus terminating the neurotransmitter signal. It is the primary site for the action of neurotoxins, such as MPTP, and the drugs of abuse, cocaine and amphetamine. It has been implicated in a number of dopamine-related disorders, including attention deficit hyperactivity disorder (ADHD), bipolar disorder, clinical depression, and alcoholism. DAT may also have a role in selective vulnerability of nigrostriatal dopaminergic neurons in Parkinson′s disease as it can transport dopamine neurotoxins.

物理的形状

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

その他情報

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 2
• 個人用保護具 (PPE): Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)