EMS0006

Recombinant Trypsin

Proteomics Grade, lyophilized powder, recombinant, expressed in Pichia pastoris

• 別名: Mass Spectrometry Trypsin, Proteomics grade Trypsin, rTrypsin
• UNSPSCコード: 12352204
• NACRES: NA.26

特性

• リコンビナント: expressed in Pichia pastoris
• 品質水準: 200
• グレード: Proteomics Grade
• フォーム: lyophilized powder
• 比活性: ≥10,000 units/mg protein
• 適合性: suitable for ; suitable for mass spectrometry
• UniProtアクセッション番号: P00761
• 輸送温度: wet ice
• 保管温度: 2-8°C

詳細

詳細

Trypsin is a major proteolytic enzyme, synthesized as a preproenzyme by pancreas and is stored as proenzyme trypsinogen in secretory granules. Trypsin belongs to the family of serine proteases that are characterized by the catalytic triad His57, Asp102 and Ser195. Trypsin is routinely used in proteomics research for peptide mapping and protein sequence work due to its highly specific cleavage resulting in a limited number of tryptic peptides. Trypsin is a pancreatic serine endoprotease which hydrolyzes peptide bonds specifically at the carboxyl side of arginine and lysine residues. The rate of hydrolysis is slower if an acidic residue is on either side of the cleavage site and cleavage may not occur if a proline residue is on the carboxyl side. The enzyme also exhibits esterase and amidase activities. Trypsin has an average molecular mass of 23.29 kDa and a pH optimum near 8.0. This product is prepared from recombinant trypsin, porcine sequence. It is naturally devoid of chymotryptic activity. This high-quality trypsin is suitable for proteomics use.

生物化学的／生理学的作用

Trypsin plays an important role in the digestion of consumed protein and contributes to the activation of other proteolytic enzymes like chemotrypsin and elastase.

安全性情報

• ピクトグラム: GHS08,GHS05,GHS07,GHS09
• シグナルワード: Danger
• 危険有害性情報: H290,H314,H317,H334,H335,H411
• 注意書き: P260 - P273 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• 危険有害性の分類: Aquatic Chronic 2 - Eye Dam. 1 - Met. Corr. 1 - Resp. Sens. 1 - Skin Corr. 1A - Skin Sens. 1 - STOT SE 3
• ターゲットの組織: Respiratory system
• 補足的ハザード: EUH071
• 保管分類コード: 8A - Combustible corrosive hazardous materials
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable