F1392

リボフラビン5'-一リン酸 ナトリウム塩 水和物

suitable for electrophoresis, suitable for acrylamide photopolymerization, ≥70%, powder

• 別名: FMN-Na, フラビンモノヌクレオチド, リボフラビン5′-リン酸 ナトリウム塩
• 実験式（ヒル表記法）: C₁₇H₂₀N₄NaO₉P · xH₂O
• CAS番号: 130-40-5
• 分子量: 478.33 (anhydrous basis)
• Beilstein: 4106529
• EC Number: 204-988-6
• MDL番号: MFCD00150992
• UNSPSCコード: 41106305
• PubChem Substance ID: 24894764
• NACRES: NA.51

特性

• 品質水準: 100
• アッセイ: ≥70%
• 形状: powder
• 光学活性: [α]20/D +37 to +42°, c = 1.5 in 5 M HCl(lit.)
• テクニック: electrophoresis: suitable
• 溶解性: H₂O: soluble 50 mg/mL, clear, orange
• &ε; （減衰係数）: 12.5 at 445 nm at 1 mM
• 適合性: suitable for acrylamide photopolymerization
• その他の活性: Protease, none detected
• 保管温度: −20°C
• SMILES記法: O.[Na+].Cc1cc2N=C3C(=O)NC(=O)N=C3N(C[C@H](O)[C@H](O)[C@H](O)COP(O)([O-])=O)c2cc1C
• InChI: 1S/C17H21N4O9P.Na.H2O/c1-7-3-9-10(4-8(7)2)21(15-13(18-9)16(25)20-17(26)19-15)5-11(22)14(24)12(23)6-30-31(27,28)29;;/h3-4,11-12,14,22-24H,5-6H2,1-2H3,(H,20,25,26)(H2,27,28,29);;1H2/q;+1;/p-1/t11-,12+,14-;;/m0../s1
• InChI Key: BHRVCJBIICJWTH-APQIITSESA-M

詳細

アプリケーション

Riboflavin 5′-monophosphate sodium salt hydrate or FMN is suitable:

• to study its mechanism of inhibition of mutagenicity of a benzo[a]pyrene 7,8-diol 9,10-epoxide, a carcinogenic metabolite of benzo[a]pyrene
• along with flavin adenine dinucleotide for reactivation of the enzyme that catalyzes the oxidation of pyridoxamine to pyridoxal in a study
• to study the reduction of polymeric azo and nitro dyes by intestinal bacteria
• to study the effect of FMN on ferrisiderophore reductase activity in the cytoplasmic fraction of Agrobacterium tumefaciens.

生物化学的／生理学的作用

FMN is a coenzyme synthesized from riboflavin (vitamin B2). It acts as a component of complex I of the electron transport chain. The enzymes containing FMN are called as flavin enzymes and are involved in oxidation-reduction mechanisms.
FMN is suitable as a photopolymerization reagent in polyacrylamide gel electrophoresis (PAGE) by forming free radicals in aqueous solution in the presence of light. FMN photodecomposes to leucoflavin. No free radicals are formed in the absence of oxygen, but traces of oxygen allow for leucoflavin to reoxidize with free-radical generation. The catalysts, tetramethylethylenediamine (TEMED) or 3-dimethylaminopropionitrile (DMAPN), are commonly added to speed up the free radical formation. Free radicals will cause acrylamide and bis-acrylamide to polymerize to form a gel matrix which can be used for sieving macromolecules. FMN is commonly used in the stacking gel for non-denaturing PAGE because native proteins can be sensitive to persulfate ions from ammonium persulfate. Another advantage of FMN over ammonium persulfate is that it will not start polymerizing until the gel is illuminated.

Riboflavin 5′-monophosphate or Flavin mononucleotide (FMN) serves as a prosthetic group for flavoenzymes. It is a part of the mitochondrial respiratory chain complex I and acts as a redox carrier. FMN is unstable in alkaline solution and is highly stable at pH 6.

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable
• 個人用保護具 (PPE): Eyeshields, Gloves, type N95 (US)