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由来生物
goat
品質水準
結合体
FITC conjugate
抗体製品の状態
affinity isolated antibody
抗体製品タイプ
secondary antibodies
グレード
flow cytometry grade
クローン
polyclonal
フォーム
buffered aqueous solution
保管条件
protect from light
テクニック
flow cytometry: 1:100
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:160
保管温度
−20°C
ターゲットの翻訳後修飾
unmodified
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詳細
IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids.
Fluorescein isothiocyanate (FITC) is a fluorescein derivative (fluorochrome) used to tag antibodies, including secondary antibodies, for use in fluorescence-based assays and procedures. FITC excites at 495 nm and emits at 521 nm.
Fluorescein isothiocyanate (FITC) is a fluorescein derivative (fluorochrome) used to tag antibodies, including secondary antibodies, for use in fluorescence-based assays and procedures. FITC excites at 495 nm and emits at 521 nm.
Mouse IgG is a plasma B cell derived antibody isotype defined by its heavy chain. IgG is the most abundant antibody isotype found in mouse serum. IgG crosses the placental barrier, is a complement activator and binds to the Fc-receptors on phagocytic cells. The level of IgG may vary with the status of disease or infection.
Anti-mouse IgG is conjugated to Fluorescein Isothiocyanate (FITC), Isomer I. The F(ab′)2 antibodies isolated from goat antiserum by affinity chromatography, react specifically with mouse IgG Fc; does not bind other mouse Igs. No cross reaction is observed with mouse Fab fragment. The antibody shows minimal cross reaction with human, horse and bovine proteins on tissue or cell preparations.
Anti-mouse IgG is conjugated to Fluorescein Isothiocyanate (FITC), Isomer I. The F(ab′)2 antibodies isolated from goat antiserum by affinity chromatography, react specifically with mouse IgG Fc; does not bind other mouse Igs. No cross reaction is observed with mouse Fab fragment. The antibody shows minimal cross reaction with human, horse and bovine proteins on tissue or cell preparations.
特異性
Goat polyclonal anti-Mouse IgG (Fc specific) F(ab′)2 fragment−FITC antibody isolated from goat antiserum by affinity chromatography reacts specifically with mouse Fc fragment. No cross reaction is observed with mouse Fab fragment. The antibodies are adsorbed to assure minimal cross reaction with human, horse and bovine proteins on tissue or cell preparations.
免疫原
Purified Fc fragments of mouse IgG
アプリケーション
Anti-Mouse IgG (Fc specific) F(ab′)2 fragment-FITC antibody produced in goat has been used in :
- flow cytometry for labelling of human peripheral blood lymphocytes (dilution of 1:100) and label human polymorphonuclear cells (PMNs) (dilution of 1:50).
- immunocytochemistry using human embryonic stem cells
- immunohistochemistry at a working dilution of 1:160.
Goat polyclonal anti-Mouse IgG (Fc specific) F(ab′)2 fragment-FITC antibody may be used as a reagent in flow cytometry, immunohistology and immunocytology offering sensitive and specific activity to mouse IgG and no cross reactivity with human, horse or bovine immunoglobulins.
Immunohistology: A minimum dilution of 1:160 is determined by indirect immunofluorescence on formalinfixed, and paraffin-embedded human tonsil using mouse monoclonal anti-human IgG as the primary antibody.
Immunohistology: A minimum dilution of 1:160 is determined by indirect immunofluorescence on formalinfixed, and paraffin-embedded human tonsil using mouse monoclonal anti-human IgG as the primary antibody.
生物化学的／生理学的作用
immunoglobulin G (IgG) antibody provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections. IgG antibody participates in complement fixation and opsonization.
その他情報
Antibody adsorbed with bovine, equine and human serum proteins.
物理的形状
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.
調製ノート
Adsorbed to reduce background with bovine, horse or human samples.
Useful when trying to avoid background staining due to the presence of Fc receptors.
Useful when trying to avoid background staining due to the presence of Fc receptors.
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
10 - Combustible liquids
WGK
nwg
引火点(°F)
Not applicable
引火点(℃)
Not applicable
個人用保護具 (PPE)
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
F2772-VAR:
F2772-BULK:
F2772-.5ML:
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試験成績書（COA）
Lot/Batch Number
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