G4040

抗Gα_i3抗体 ウサギ宿主抗体

whole antiserum, buffered aqueous solution

• 別名: Anti-87U6, Anti-ARCND1, Anti-HG1A
• MDL番号: MFCD00239679
• UNSPSCコード: 12352203
• NACRES: NA.41
• 結合体: unconjugated
• application: IP; WB
• クローン: polyclonal
• 化学種の反応性: mouse, human, rat, hamster
• citations: 12
• テクニック: immunoprecipitation (IP): suitable; western blot: 1:1,000-1:2,000 using solubilized mouse and rat brain membrane lysates, and mouse 3T3 cell lysates

特性

• 由来生物: rabbit
• 品質水準: 200
• 結合体: unconjugated
• 抗体製品の状態: whole antiserum
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• フォーム: buffered aqueous solution
• 分子量: antigen ~40 kDa
• 化学種の反応性: mouse, human, rat, hamster
• テクニック: immunoprecipitation (IP): suitable; western blot: 1:1,000-1:2,000 using solubilized mouse and rat brain membrane lysates, and mouse 3T3 cell lysates
• UniProtアクセッション番号: P08754
• 輸送温度: wet ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... GNAI3(2773) ; mouse ... Gnai3(14679) ; rat ... Gnai3(25643)

詳細

詳細

G-proteins are membrane associated heterotrimeric proteins that are comprised of α-, β-, and λ-subunits. These proteins play a key role in signal transduction pathways that regulate cell′s response to several hormones, neuromodulators and ligands.

特異性

Gα_i3およびGα₀型Gタンパク質を認識します。

免疫原

Gα_i3のC末端領域に相当する合成ペプチド。

アプリケーション

Anti-Gα i3 antibody produced in rabbit is suitable for immunoprecipitation at a working antibody amount of 4μL using solubilized mouse brain membrane lysates and immunoblotting at a working dilution of 1:2000 using solubilized mouse and rat brain membrane lysates, and mouse 3T3 cell lysates.

生物化学的／生理学的作用

Gi α 3 is a G-protein subunit that is involved in many signal transduction pathways including the mediation of EGF-induced PLC-γ activation and Ca⁺² mobilization in hepatocytes. The α-subunit contains a guanine-binding domain that is in its inactive state when it is occupied by GDP. Upon activation, GDP is replaced with GTP, causing the dissociation of the α-subunit from the βλ-subunit complex. This enables the Gα-GTP complex to bind to and regulate specific signaling pathways. GTP is then hydrolyzed, allowing for re-association of the α-subunit with the βλ-subunit complex.

物理的形状

Solution of whole antiserum diluted in PBS, pH 7.4.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable