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グレード
for molecular biology
フォーム
plate
保管温度
2-8°C
詳細
The HIS-Select® Filter Plate is an immobilized metal-ion affinity chromatography (IMAC) product that allows rapid purification of small-scale crude cell extracts containing histidine-tagged proteins. The HIS-Select® Filter Plate contains 20 μm, spherical silica particles (100 nm pore size) with a hydrophilic layer.
The HIS-Select® Filter Plate is selective for recombinant proteins with histidine tags and exhibits very low non-specific binding of other proteins. The selectivity can be modulated with the inclusion of imidazole during chromatography. The binding capacity of the HIS-Select® Filter Plate is typically > 2 mg per well as determined with an approximately 30 kDa histidine-tagged protein.
The HIS-Select® Filter Plate is selective for recombinant proteins with histidine tags and exhibits very low non-specific binding of other proteins. The selectivity can be modulated with the inclusion of imidazole during chromatography. The binding capacity of the HIS-Select® Filter Plate is typically > 2 mg per well as determined with an approximately 30 kDa histidine-tagged protein.
アプリケーション
The HIS-Select® Filter Plate is suitable for purification of recombinant proteins with histidine tags.
特徴および利点
- High Selectivity for his-tagged proteins
- Ready-to-use plate format
- Compatible with CelLytic™ cell lysis reagents
法的情報
米国特許番号6,623,655によって保護されており、研究目的の使用に限り販売しています。商用目的の場合は、別途ライセンスを取得する必要があります。
CelLytic is a trademark of Sigma-Aldrich Co. LLC
HIS-Select is a registered trademark of Merck KGaA, Darmstadt, Germany
関連製品
製品番号
詳細
価格
シグナルワード
Danger
危険有害性の分類
Aquatic Chronic 3 - Carc. 1A Inhalation - Muta. 2 - Repr. 1B - Resp. Sens. 1 - Skin Sens. 1 - STOT RE 1 - STOT RE 2 Inhalation
ターゲットの組織
Respiratory Tract
保管分類コード
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
WGK
WGK 3
引火点(°F)
Not applicable
引火点(℃)
Not applicable
最新バージョンのいずれかを選択してください：
Benjamin D Allen et al.
Proceedings of the National Academy of Sciences of the United States of America, 107(46), 19838-19843 (2010-11-04)
The stability, activity, and solubility of a protein sequence are determined by a delicate balance of molecular interactions in a variety of conformational states. Even so, most computational protein design methods model sequences in the context of a single native
Isabel K Macdonald et al.
PloS one, 7(7), e40759-e40759 (2012-07-21)
An assay employing a panel of tumor-associated antigens has been validated and is available commercially (EarlyCDT®-Lung) to aid the early detection of lung cancer by measurement of serum autoantibodies. The high throughput (HTP) strategy described herein was pursued to identify
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