H4149

モノクローナル抗熱ショックタンパク質60抗体 マウス宿主抗体

clone LK1, ascites fluid

• MDL番号: MFCD02189138
• UNSPSCコード: 12352203
• NACRES: NA.41
• 結合体: unconjugated
• application: ELISA (i); EM; IHC (p); WB
• クローン: LK1, monoclonal
• 化学種の反応性: rat, human, chicken
• citations: 37
• テクニック: electron microscopy: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; indirect ELISA: suitable; western blot: 1:400 using cultured human foreskin fibroblast extract

特性

• 由来生物: mouse
• 品質水準: 200
• 結合体: unconjugated
• 抗体製品の状態: ascites fluid
• 抗体製品タイプ: primary antibodies
• クローン: LK1, monoclonal
• 分子量: antigen 60 kDa
• 含みます: 15 mM sodium azide
• 化学種の反応性: rat, human, chicken
• テクニック: electron microscopy: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; indirect ELISA: suitable; western blot: 1:400 using cultured human foreskin fibroblast extract
• アイソタイプ: IgG1
• UniProtアクセッション番号: P10809
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... HSPD1(3329) ; rat ... Hspd1(63868)

詳細

詳細

Heat shock (stress) proteins (HSP) are produced by prokaryotic and eukaryotic cells, and are among the most conserved molecules in phylogeny. HSP-60 is a member of HSP family with a uniquely high level of sequence conservation.

Monoclonal Anti-Heat Shock Protein 60 (mouse IgG1 isotype) is derived from the LK1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with recombinant human heat diseases.

特異性

本抗体は、ヒトHSP60のアミノ酸残基383-447の間に位置するエピト-プを認識します。細菌性(E. coli)の相当分子や蠕虫、ホウレンソウのHSP60に対する交差反応性はありません。本抗体は構成型および誘導型のHSP60と反応します。若年性慢性関節炎患者から採取された滑膜の免疫組織化学において、高レベルで染色されている様子が示されています。

免疫原

リコンビナントヒト熱ショックタンパク質60(HSP60)。

アプリケーション

Monoclonal Anti-Heat Shock Protein 60 has been used in the following techniques:

• Enzyme linked immunosorbent assay (ELISA)
• Immunoblotting
• Immunocytochemistry
• Immunohistochemistry
• Immunoelectron microscopy
• Double immunofluorescence

生物化学的／生理学的作用

A wide variety of environmental perturbations, such as a sudden increase in temperature, induce cells to rapidly synthesize a group of polypeptides known as the heat shock (stress) proteins. HSP-60 is considered to be a potential antigen in a number of autoimmune diseases. Abnormal immune reactivity involving shock protein 60 (HSP60) has also been implicated in the pathogenesis of schizophrenia. This monoclonal antibody enables the differentiation between the HSP60 of mammalian and bacterial origin.

Abnormal immune reactivity involving HSP60 has also been implicated in the pathogenesis of schizophrenia.

物理的形状

Monoclonal Anti-Heat Shock Protein 60 is provided as ascites fluid with 0.1% sodium azide as a preservative.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: nwg
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable