J4750

モノクロナール抗JNK, 活性化 (二リン酸化型JNK) マウス宿主抗体

clone JNK-PT48, purified from hybridoma cell culture

• 別名: モノクロナール抗JNK, 活性化 (二リン酸化型JNK)
• UNSPSCコード: 12352203
• NACRES: NA.44

特性

• 由来生物: mouse
• 品質水準: 200
• 結合体: unconjugated
• 抗体製品の状態: purified from hybridoma cell culture
• 抗体製品タイプ: primary antibodies
• クローン: JNK-PT48, monoclonal
• 形状: buffered aqueous solution
• 分子量: antigen 46 kDa; antigen 54 kDa
• 化学種の反応性: mouse, human, rat
• 濃度: ~2 mg/mL
• テクニック: immunocytochemistry: suitable; indirect ELISA: suitable; microarray: suitable; western blot: 4-20 μg/mL using extract of human Jurkat cells activated with anisomycin
• アイソタイプ: IgG1
• UniProtアクセッション番号: P45983
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... MAPK8(5599) ; mouse ... Mapk8(26419) ; rat ... Mapk8(116554)

詳細

詳細

Monoclonal Anti-JNK, Activated (Diphosphorylated JNK) (mouse IgG1 isotype) is derived from the JNK-PT48 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to the phosphorylated form of JNK activation loop, conjugated to KLH. JNK (c-Jun N terminal protein kinase) belongs to the mitogen-activated protein kinase (MAPK) superfamily of enzymes. The kinases in the MAPK level are activated by phosphorylation of both tyrosine (Y) and threonine (T) residues organized in a TXY motif. The residue in between the two phosphorylated residue determines the specificity of activation of the MAPKs and is proline for JNK. JNK1-3 of molecular weights 46, 54, and 52 kDa respectively.

特異性

二リン酸化型の活性化JNKと特異的に反応します。本抗体が認識するエピト-プには、JNKの調節部位(Thr¹⁸³, Tyr¹⁸⁵)が含まれています。 スレオニン一リン酸化型のペプチドとわずかな交差反応性が観察されていますが、非リン酸化型やチロシン一リン酸化型のJNKペプチドとは反応しません。 p38-MAPKやERK-MAPKの場合、リン酸化の有無にかかわらず認識しません。

免疫原

リン酸化型JNKの活性化ル-プに相当するアミノ酸pTPpYVVTRYYRを含む配列の合成ペプチド。

アプリケーション

Monoclonal Anti-JNK, Activated (Diphosphorylated JNK) antibody has been used in immunoblotting, enzyme linked immunosorbent assay (ELISA), immunocytochemistry, western blotting, immunohistochemistry.

生物化学的／生理学的作用

c-Jun N terminal protein kinase (JNK) is mostly stress-responsive kinase and have been implicated in cell death in several cellular systems. The JNK cascade is triggered by small GTPases that are Rac and Cell division control protein 42 (CDC42). After activation, JNKs translocate to the nucleus where they physically associate with and activate their target transcription factors (cJun, AMP-dependent transcription factor (ATF) etc.).

物理的形状

PBS溶液 (pH 7.4, 15mMアジ化ナトリウム含有)。

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: nwg
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable