M0321

MDM2 ヒト

recombinant, expressed in E. coli, buffered aqueous glycerol solution, ≥75% (SDS-PAGE)

• UNSPSCコード: 12352202
• NACRES: NA.77

特性

• 由来生物: human
• 品質水準: 200
• リコンビナント: expressed in E. coli
• 詳細: contains an N-terminal GST tag
• アッセイ: ≥75% (SDS-PAGE)
• 形状: buffered aqueous glycerol solution
• 分子量: ~84 kDa
• UniProtアクセッション番号: Q00987
• 輸送温度: dry ice
• 保管温度: −70°C
• 遺伝子情報: human ... MDM2(4193)

詳細

詳細

Murine double minute 2 (MDM2) encoding gene is localized at 12q15 in the human chromosome. It is a multidomain nuclear phosphoprotein. It possesses really interesting new gene (RING) finger domain and its function and structural integrity is required for the action of MDM2. It also has central acidic domain.

生物化学的／生理学的作用

Murine double minute 2 (MDM2) is a chief regulator of the p53 tumor suppressor protein. It is a multifunctional oncoprotein that contributes to both p53-dependent and -independent roles in oncogenesis. MDM2 has various p53-independent functions that contribute to its activity as an oncogene.

Overexpression of Murine double minute 2 (MDM2) is observed in various cancer like lung, gastric, colorectal, hepatocellular, breast, endometrial, and prostate. A common single nucleotide polymorphism is observed at 309 amino acid (from T to G) increases the susceptibility to cancer.

MDM2 is a negative regulator of the p53 tumor suppressor.

Mdm2 functions as an E3 ubiquitin ligase and an inhibitor of p53 transcriptional activation.

物理的形状

50 mM Tris-HCl溶液(pH 7.5)、50 mM NaCl、0.25 mM DTT、0.1 mM PMSF、25%グリセロールで調製した溶液。

調製ノート

N-末端GST融合短縮形(202アミノ酸～N末端)。

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable
• 個人用保護具 (PPE): Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)