P2859

モノクロナール抗p300/CBP マウス宿主抗体

clone NM11, purified from hybridoma cell culture

• 別名: 抗CREB結合タンパク質抗体
• MDL番号: MFCD02097119
• UNSPSCコード: 12352203
• NACRES: NA.41
• 結合体: unconjugated
• application: ARR; ICC; IP; WB
• クローン: NM11, monoclonal
• 化学種の反応性: mouse, rat, human, mink, monkey
• citations: 15
• テクニック: immunocytochemistry: suitable; immunoprecipitation (IP): suitable; microarray: suitable; western blot: 10-20 μg/mL using human 293 embryonal kidney cells

特性

• 由来生物: mouse
• 品質水準: 200
• 結合体: unconjugated
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: NM11, monoclonal
• フォーム: buffered aqueous solution
• 分子量: antigen 300 kDa
• 化学種の反応性: mouse, rat, human, mink, monkey
• 濃度: ~2 mg/mL
• テクニック: immunocytochemistry: suitable; immunoprecipitation (IP): suitable; microarray: suitable; western blot: 10-20 μg/mL using human 293 embryonal kidney cells
• アイソタイプ: IgG1
• UniProtアクセッション番号: Q92831
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... KAT2B(8850) ; mouse ... Kat2b(18519) ; rat ... Pcaf(301164)

詳細

詳細

Monoclonal Anti-p300/CBP (mouse IgG1 isotype) is derived from the NM11 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a native human p300. The KAT2B (lysine acetyltransferase 2B) gene encodes a p300/CBP (CREB binding protein)-associated factor, which belongs to the family of GNAT (GCN5 (general control nonderepressible 5)-related N-acetyltransferase).

特異性

p300およびCBPと特異的に反応しますが、関連するp270分子とは反応しません。エピト-プは、CBPのC末端付近のアミノ酸2071-2091の21アミノ酸内に存在します。CBPとp300は、この21アミノ酸領域内の隣接しない３残基が異なりますが、検出範囲では抗体の反応性には影響しません。

免疫原

天然型ヒトp300。

生物化学的／生理学的作用

p300/CBP are capable of binding to a variety of transcriptional activator and regulatory molecules, including p53 and nuclear hormone receptors. The complexity of these p300/CBP cellular associations suggests that both proteins play a central role in the coordination of gene expression during cell growth and differentiation.
KAT2B (lysine acetyltransferase 2B) possess histone acetyltransferase (HAT) activity and is thus known to regulate transcription process. Acetylation also influences the chromatin structure. Lysine acetylation significantly contributes to protein modification. It is essential for establishing protein function by altering its structure, activity and molecular interaction.

物理的形状

0.01M PBS溶液 (pH 7.4, 15mMアジ化ナトリウム含有)

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: nwg
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable