P6746

モノクローナル抗PML抗体 マウス宿主抗体

clone PML-97, purified from hybridoma cell culture

• 別名: 抗前骨髄球性白血病抗体
• MDL番号: MFCD06412254
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 200
• 結合体: unconjugated
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: PML-97, monoclonal
• 形状: buffered aqueous solution
• 分子量: antigen ~90 kDa
• 化学種の反応性: hamster, human, mouse
• テクニック: immunocytochemistry: suitable; immunoprecipitation (IP): suitable; indirect ELISA: suitable; microarray: suitable; western blot: 1-2 μg/mL using 293T cells expressing recombinant PML3
• アイソタイプ: IgG1
• UniProtアクセッション番号: P29590
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... PML(5371)

詳細

詳細

Monoclonal Anti-PML (mouse IgG1 isotype) is derived from the PML-97 hybridoma produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with recombinant full-length human PML (promyelocytic leukemia) protein. PML is characterized with cysteine-rich zinc-binding domains (RING), two B-boxes, an adjacent leucine coiled coil forming the RBCC or tripartite motif (TRIM), and a nuclear localization signal (NLS). The RING and B-box are important for the interaction of the PML protein with different proteins. In normal cells, the PML protein is found in nuclear bodies (PML NBs) whereas in acute promyelocytic leukemia (APL) patients, the PML NBs are disrupted and delocalized from PML NBs to nucleus and cytoplasm.

The PML gene is located on the human chromosome at 15q24.1.

特異性

Monoclonal Anti-PML recognizes human, mouse, and hamster PML (about 90 kDa)

免疫原

リコンビナントヒト完全長PML(前骨髄球性白血病)タンパク質。

アプリケーション

Anti-PML antibody, Mouse monoclonal may be used in:

• enzyme-linked immunosorbent assay (ELISA)
• immunoblotting
• immunoprecipitation
• immunocytochemistry
• indirect immunofluorescence

生物化学的／生理学的作用

A monoclonal antibody to promyelocytic leukemia (PML) is an important tool for studying PML function in normal and cancerous cells. In normal cells, the PML protein and the PML nuclear bodies play a role in different physiological processes such as: growth control, transcription, DNA repair, DNA replication and RNA transport.

物理的形状

0.01M PBS溶液 (pH 7.4, 1%BSA, 15mMアジ化ナトリウム含有)。

保管および安定性

For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: nwg
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable
• 個人用保護具 (PPE): Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)