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詳細
ProteoPrep® Total Extraction Sample Kit is designed to extract all proteins from any type of cell or tissue. It includes two reagents that have been used extensively for extraction and two new reagents with increased solubilizing power to extract more proteins. This kit also includes reagents for the reduction and alkylation of disulfide bonds. This allows comparison of the protein extractions obtained with each of the four reagents and optimization to meet your individual needs. The special reducing and alkylating reagents produce protein samples that exhibit improved focusing and decreased streaking in 2D gels.
アプリケーション
ProteoPrep® Total Extraction Sample Kit has been used to extract total protein from various sources such as epidermal stem cells and rheumatoid arthritis (RA) synovial fibroblasts.
特徴および利点
- Four pre-mixed solubilization reagents - Enables rapid solubilization.
- Pre-measured reducing and alkylating reagents - Easy-to-use reagents provide improved IEF resolution.
- Innovative detergent preparations - Highly improved solubility allows higher protein loads and greater visibility of low abundance proteins in 2D gels.
保管および安定性
These reagents should remain stable for at least 6 months in their unopened container.
その他情報
The ProteoPrep® Total Extraction Kit was designed through a collaboration of Proteome Systems and Sigma research scientists.
This kit contains enough reagents to generate at least ten 2-ml samples using all four reagents. For researchers who have optimized an extraction protocol using one chaotropic extraction reagent, each reagent is available as an individual product as well.
This kit contains enough reagents to generate at least ten 2-ml samples using all four reagents. For researchers who have optimized an extraction protocol using one chaotropic extraction reagent, each reagent is available as an individual product as well.
法的情報
ProteoPrep is a registered trademark of Merck KGaA, Darmstadt, Germany
免責事項
Samples containing high amount of salts and buffers may not work with this kit. These samples should be dialyzed prior to use. This product is for R&D use only, not for drug, household, or other uses.
キットの構成要素は別途購入可能です。
製品番号
詳細
SDS
- C0481Protein Extraction Reagent Type 1SDS
- C0606Protein Extraction Reagent Type 2SDS
- C0731Protein Extraction Reagent Type 3SDS
- C0356Protein Extraction Reagent Type 4SDS
- A3221Iodoacetamide, Single use vial of 56 mg 5 x 56SDS
- T7567Tributylphosphine solution, 200 mM (in N-methyl-2-pyrrolidinone), liquid 5 x 0.5SDS
シグナルワード
Danger
危険有害性の分類
Acute Tox. 3 Oral - Aquatic Chronic 2 - Carc. 2 - Eye Dam. 1 - Repr. 1B - Resp. Sens. 1 - Skin Corr. 1A - Skin Sens. 1 - STOT SE 3
ターゲットの組織
Respiratory system
保管分類コード
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
毒物及び劇物取締法
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PRTR
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消防法
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労働安全衛生法名称等を表示すべき危険物及び有害物
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労働安全衛生法名称等を通知すべき危険物及び有害物
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カルタヘナ法
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Jan Code
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We have developed a method for on-membrane direct identification of phosphoproteins, which are detected by a phosphate-binding tag (Phos-tag) that has an affinity to phosphate groups with a chelated Zn2+ ion. This rapid profiling approach for phosphoproteins combines chemical inkjet
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The main cause of steroid-induced necrosis of femoral head (SNFH) is excessive glucocorticoid (GC) intake. The aim of this article was to investigate the role of lncRNA NEAT1 as a molecular sponge to adsorb miR-23b-3p and regulate CYP1A2 in SNFH.
Brucine inhibits TNF-a-induced HFLS-RA cell proliferation by activating the JNK signaling pathway
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Experimental and Therapeutic Medicine (2019)
Effects of porcine acellular dermal matrix
treatment on wound healing and scar formation:
Role of Jag1 expression in epidermal stem cells
treatment on wound healing and scar formation:
Role of Jag1 expression in epidermal stem cells
Chen Xiao-Dong, et al.
Organogenesis (2018)
Selim Ceylan et al.
Canadian journal of microbiology, 57(4), 295-302 (2011-04-16)
A gram-negative, moderately halophilic bacterium was isolated from Çamaltı Saltern area, located in the Aegean Region of Turkey. Analysis of its 16S rRNA gene sequence and physiological characteristics showed that this strain belonged to the genus Halomonas ; hence, it
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