S9822

Anti-Sonic Hedgehog peptide (C-terminal) antibody produced in goat

affinity isolated antibody, lyophilized powder

• 別名: Anti-Dsh, Anti-Hhg1, Anti-Hx, Anti-Shh
• UNSPSCコード: 51111800
• NACRES: NA.41
• 結合体: unconjugated
• application: IHC; WB
• クローン: polyclonal
• 化学種の反応性: mouse
• citations: 5
• テクニック: immunohistochemistry: 15 μg/mL; western blot: 0.1-0.2 μg/mL

特性

• 由来生物: goat
• 品質水準: 100
• 結合体: unconjugated
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• フォーム: lyophilized powder
• 化学種の反応性: mouse
• テクニック: immunohistochemistry: 15 μg/mL; western blot: 0.1-0.2 μg/mL
• UniProtアクセッション番号: Q62226
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: mouse ... Shh(20423)

詳細

詳細

The Hedgehog (Hh) family of secreted proteins consists of sonic hedgehog (Shh), desert hedgehog (Dhh) and Indian hedgehog (Ihh). The Hh signaling regulates cell migration and tissue construction during embryogenesis and reconstruction in adults. Shh protein is secreted by Purkinje cells in the cerebellum with both autocrine and paracrine functions. The Shh signaling is mediated by the interaction of Shh with transmembrane proteins, Patched and Smoothened. The Shh pathway cross-talks Notch and Wnt pathways and acts via downstream targets such as cyclins, γ catenin, myc and other transcription factors. The Hh family members have multiple roles as morphogens, cell survival factors, setting anterior-posterior axis and digit formation during development of limb and axon guidance factors during the development of brain and nervous system. Hedgehog signaling has been implicated in childhood tumors of pancreas, liver, thyroid, gastrointestinal tract, neuroblastomas and medulloblastomas
Anti-Sonic Hedgehog Peptide (C-terminal) recognizes mouse Sonic Hedgehog C-terminal peptide. This antibody shows less than 5% cross-reactivity with rmDhh C-terminal peptide and less than 1% cross-reactivity with a 6X histidine-tagged rmShh N-terminal peptide (amino acids 25-198).

免疫原

purified, E. coli-derived recombinant mouse 6× histidine-tagged sonic hedgehog C-terminal peptide.

アプリケーション

Anti-Sonic Hedgehog Peptide (C-terminal) can be used for immunoblotting at a working concentration of 0.1-0.2 μg/ml. For immunohistochemistry, a working concentration of 15 μg/ml is recommended.

物理的形状

凍結乾燥品(PBS溶液, 5%トレハロース含有, 0.2 μmフィルター滅菌済み)。

安全性情報

• 保管分類コード: 13 - Non Combustible Solids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable
• 個人用保護具 (PPE): Eyeshields, Gloves, type N95 (US)