SAB4200357

Anti-Profilin 1 antibody, Mouse monoclonal

clone Profilin 1-3, purified from hybridoma cell culture

• 別名: Anti-PFN1
• UNSPSCコード: 12352203
• NACRES: NA.41
• 結合体: unconjugated
• application: IP; WB
• クローン: Profilin 1-3, monoclonal
• 化学種の反応性: rat, bovine, human, mouse, canine
• citations: 5
• テクニック: immunoprecipitation (IP): suitable; western blot: 1-2 μg/mL using whole extracts of HeLa or NRK cells

特性

• 由来生物: mouse
• 品質水準: 200
• 結合体: unconjugated
• 抗体製品の状態: purified from hybridoma cell culture
• 抗体製品タイプ: primary antibodies
• クローン: Profilin 1-3, monoclonal
• フォーム: buffered aqueous solution
• 分子量: antigen ~15 kDa
• 化学種の反応性: rat, bovine, human, mouse, canine
• 濃度: ~1.0 mg/mL
• テクニック: immunoprecipitation (IP): suitable; western blot: 1-2 μg/mL using whole extracts of HeLa or NRK cells
• アイソタイプ: IgG1
• UniProtアクセッション番号: P07737
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... PFN1(5216) ; mouse ... Pfn1(18643) ; rat ... Pfn1(64303)

詳細

詳細

Monoclonal Anti-Profilin 1 (mouse IgG1 isotype) is derived from the hybridoma Profilin1-3 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to a sequence located near the C-terminus of human profiling 1, conjugated to keyhole limpet hemocyanin (KLH). Profilin-1 (Pfn1), a major actin-regulatory protein is mapped to human chromosome17p13. It is the most ubiquitous and abundant protein and is highly expressed throughout development and adulthood in most tissues including brain.

免疫原

synthetic peptide corresponding to a sequence located near the C-terminus of human profilin 1, conjugated to KLH. The corresponding sequence is identical in mouse and rat profilin 1.

アプリケーション

Monoclonal Anti-Profilin 1 antibody produced in mouse has been used in immunoblotting and immunoprecipitation.

生物化学的／生理学的作用

Profilin-1 (Pfn1) is involved in actin polymerization in response to extracellular signals. Profilins were shown to be important for normal cell proliferation, differentiation and motility. Profilin 1 is a potent regulator of actin filament dynamics. It is suggested to act as a tumor suppressor protein based on its reduced expression in several types of invasive cancers and its ability to suppress tumorigenicity when over expressed in breast cancer cells. Deletion of the profilin 1 gene leads to an embryonic lethal phenotype.

物理的形状

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable