P7624

抗プロフィリン1 (C-末端) ウサギ宿主抗体

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

• 別名: Anti-PFN1
• MDL番号: MFCD09265368
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: rabbit
• 品質水準: 200
• 結合体: unconjugated
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 形状: buffered aqueous solution
• 分子量: antigen ~15 kDa
• 化学種の反応性: mouse, human, rat
• 濃度: ~1 mg/mL
• テクニック: immunoprecipitation (IP): 5-10 μg using mouse NIH3T3 cell lysates; indirect immunofluorescence: 10-20 μg/mL using rat NRK cells; western blot (chemiluminescent): 1-2 μg/mL using whole extract of human HeLa cells
• UniProtアクセッション番号: P15735
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... PFN1(5216) ; mouse ... Pfn1(18643) ; rat ... Pfn1(64303)

詳細

詳細

Profilin 1 is a ubiquitous actin monomer-binding protein. Profilin 1 is the most ubiquitous and abundant and is highly expressed throughout development and adulthood in most tissues including brain.

免疫原

synthetic peptide corresponding to amino acid residues 128-140 of human profilin 1, conjugated to KLH. The corresponding sequence is identical in rat and mouse.

アプリケーション

Anti-Profilin 1 (C-terminal) antibody produced in rabbit has been used in:

• immunoblotting
• immunostaining
• immunoprecipitation
• immunofluorescence
• profilin enzyme-linked immunosorbent assay (ELISA) analysis

生物化学的／生理学的作用

Profilin 1 is involved in actin polymerization in response to extracellular signals. Profilins were shown to be important for normal cell proliferation, differentiation and motility. Deletion of profilin 1 gene leads to an embryonic lethal phenotype. Profilin 1 is a potent regulator of actin filament dynamics. Although profilin 1 prevents spontaneous actin polymerization by complexing with unpolymerized actin in vivo, actin-profilin complexes can be added to free barbed ends, thereby stimulating actin polymerization. Profilin 1 was suggested to act as a tumor suppressor protein based on its reduced expression in several types of invasive cancers and its ability to suppress tumorigenicity when over-expressed in breast cancer cells. Deletion of profilin 1 is associated with Miller-Dieker syndrome.

ターゲットの説明

Profilin 1 is a ubiquitous actin monomer-binding proteininvolved in actin polymerization in response toextracellular signals.

物理的形状

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable
• 個人用保護具 (PPE): Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)