P0101

抗プロフィリン 2 (C末端) 抗体 ウサギ宿主抗体

IgG fraction of antiserum, buffered aqueous solution

• 別名: 抗PFN2抗体
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: rabbit
• 品質水準: 200
• 結合体: unconjugated
• 抗体製品の状態: IgG fraction of antiserum
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• フォーム: buffered aqueous solution
• 分子量: antigen ~14 kDa
• 交差性: rat, mouse, human
• 包装: antibody small pack of 25 μL
• テクニック: western blot: 1:500-1:1,000 using whole extracts of human SH-SY5Y cells, mouse brain, and rat brain
• UniProtアクセッション番号: P35080
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... PFN2(5217) ; mouse ... Pfn2(18645) ; rat ... Pfn2(81531)

詳細

詳細

Profilins are actin-monomer-binding proteins, abundantly expressed in all eukaryotic cells from yeast to mammals. Profilin 2 (PFN2) is the neuronal specific isoform. It is an actin-binding protein and is a member of the small G-actin-binding class of protein.

免疫原

ヒトプロフィリン2のアミノ酸128~140に相当する合成ペプチドのKLH結合体。 相当する配列はマウスおよびラットと同一です。

アプリケーション

Anti-Profilin 2 (C-terminal) antibody produced in rabbit has been used in immunostaining and immunoblotting.

生物化学的／生理学的作用

Profilins are key regulators of actin dynamics. Profilin 2 (PFN2) associates with a number of proteins involved in membrane trafficking and endocytosis, such as synapsin and dynamin 1. It associates with dynamin 1 through the C-terminal proline-rich domain of dynamin 1 and thereby competes with the binding of SH3 ligands, such as endophilin and amphiphysin, thus interfering with the assembly of the endocytic machinery. Overexpression of profilin 2 inhibits endocytosis. Absence of profilin 2 in neurons increases endocytosis and membrane recycling. Profilin 2 is required for stimulated actin polymerization in the synapse and is involved in the regulation of synaptic vesicle exocytosis, neuronal excitability and complex behavior.

物理的形状

0.01 M PBS溶液 (pH 7.4, 15 mM アジ化ナトリウム含有)。

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable
• 個人用保護具 (PPE): Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)