SAB4504332

Anti-phospho-Akt (pThr³⁰⁸) antibody produced in rabbit

affinity isolated antibody

• UNSPSCコード: 12352203
• NACRES: NA.41
• 結合体: unconjugated
• application: ELISA; IHC; WB
• クローン: polyclonal
• 化学種の反応性: mouse, rat, human
• citations: 7
• テクニック: ELISA: 1:10000; immunohistochemistry: 1:50-1:100; western blot: 1:500-1:1000

特性

• 由来生物: rabbit
• 品質水準: 100
• 結合体: unconjugated
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• フォーム: buffered aqueous solution
• 分子量: antigen 55 kDa
• 化学種の反応性: mouse, rat, human
• 濃度: ~1 mg/mL
• テクニック: ELISA: 1:10000; immunohistochemistry: 1:50-1:100; western blot: 1:500-1:1000
• NCBIアクセッション番号: P31749; P31751
• UniProtアクセッション番号: P31751
• 輸送温度: wet ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: phosphorylation (pThr308)
• 遺伝子情報: human ... AKT1(207) , AKT2(208)

詳細

詳細

The AKT/protein kinase B gene has three homologues, namely PKBα (Akt1), PKBβ (Akt2), and PKBγ (Akt3), which are localized to human chromosome 14q32, 19q13, and 1q44, respectively. The members of AKT family contain an amino-terminal pleckstrin homology (PH) domain, a short α-helical linker, and a carboxyl-terminal kinase domain. The AKT1 and AKT2 genes are expressed highly in insulin-responsive tissues, such as brown fat.

免疫原

The antiserum was produced against synthesized peptide derived from human Akt around the phosphorylation site of Thr308.

Immunogen Range: 276-325

アプリケーション

Anti-phospho-Akt (pThr308) antibody produced in rabbit has been used in immunoblotting.

生物化学的／生理学的作用

AKT is activated in a multistep process that involves its translocation across the membrane and its phosphorylation. The 3′-phosphorylated phosphoinositides, 3,4,5-trisphosphate (PI-3,4,5-P3) and PI-3,4,-P2 produced by PI3K bind to the PH (pleckstrin homology) domain and this binding facilitates the localization of AKT kinases to the plasma membrane. Once localized, PDK1 (3-phosphoinositide-dependent kinase) phosphorylates Thr-308/309 residue on the AKT molecule, which is essential for its activation. Another residue, Ser- 473/474 on AKT, is also phosphorylated by PDK2. This phosphorylation, although not necessary, increases the activity of AKT. It plays a role in cell survival by regulating the effect of growth factors. AKT may facilitate phosphorylation and inactivation of glycogen synthase kinase-3 by insulin. In adipocytes, it may be involved in the activation of glucose transporter translocation. It is involved in the regulation of several proteins that are involved in cellular processes, such as metabolism, apoptosis, and proliferation. Variations in PI3K-AKT signaling have been observed in several types of cancer, such as human gastric cancer, prostate and breast cancers.

特徴および利点

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物理的形状

ウサギIgGのPBS溶液（Mg2+およびCa2+を含まず）、pH 7.4、150 mM NaCl、0.02％ アジ化ナトリウム、50％ グリセロール

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: nwg
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable