SAE0078

MMP-9 pre-activated human

recombinant, ≥1,300 pmol/min/μg, expressed in HEK 293 cells

• 別名: MMP-9
• Enzyme Commission number: 3.4.24.35 (BRENDA, IUBMB)
• UNSPSCコード: 12352202
• NACRES: NA.32

特性

• 由来生物: human
• 品質水準: 200
• リコンビナント: expressed in HEK 293 cells
• アッセイ: ≥95% (SDS-PAGE)
• 形状: liquid
• 分子量: calculated mol wt 66 kDa; observed mol wt 82 kDa by SDS-PAGE (The protein migrates as a 82 kDa protein on SDS-PAGE due to glycosylation)
• 濃度: ≥50 μg/mL
• UniProtアクセッション番号: P14780
• アプリケーション: cell analysis
• 輸送温度: dry ice
• 保管温度: −20°C
• 遺伝子情報: human ... MMP(4318)

詳細

詳細

50 μg protein, determined by Bradford. The protein concentration is ≥50 μg/ml. for the lot-specific concentration, see Certificate of Analysis.

生物化学的／生理学的作用

MMP-9 is a member of the matrix metalloproteinase (MMP) family of proteins. Proteins of the MMP family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Studies in rhesus monkeys suggest that MMP9 is involved in IL-8 (interleukin-8)-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. Thrombospondins, intervertebral disc proteins, regulate the effective levels of MMP-2 and -9, which are key effectors of extracellular matrix (ECM) remodeling. MMP-9 degrades various substrates including gelatin, collagen types IV and V, and elastin. MMP-9 is involved in a variety of autoimmune diseases such as systemic lupus erythematosus, rheumatoid arthritis, and multiple sclerosis, and be regarded as a potential therapeutic target.
As with most MMPs, MMP-9 is secreted as an inactive pro-protein which is activated when cleaved by extracellular proteinases. MMP-9 can be cleaved (and thus activated) in vitro using 4-Aminophenylmercuric acetate (APMA), Cat. No. A9563. MMP-9 is secreted from neutrophils, macrophages, and a number of transformed cells, and is the most complex family member in terms of domain structure and regulation of its activity.
Structurally, MMP9 maybe be divided into five distinct domains: a pro-domain which is cleaved upon activation, a gelatin binding domain consisting of three contiguous fibronectin type II units, a catalytic domain containing the zinc binding site, a proline rich linker region, and a carboxyl terminal hemopexin like domain.
As with most MMPs, MMP-9 is secreted as an inactive pro-protein which is activated when cleaved by extracellular proteinases. This product was cleaved and activated in vitro using 4-Aminophenylmercuric acetate (APMA), Cat. No. A9563.

その他情報

This product was pre-activated in vitro using 4-Aminophenylmercuric acetate (APMA). Thus, it is active and ready for use. In order to save our customers from handling hazardous materials, and for environmental saving, the highly toxic and fatal APMA was removed from the final preparation.

物理的形状

Liquid solution, 0.22 mm filtered, containing 25 mM Tris, 10 mM CaCl2, 0.05% Brij-35

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable