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リコンビナント
expressed in human cells
expressed in insect cells
UniProtアクセッション番号
輸送温度
dry ice
保管温度
−70°C
遺伝子情報
human ... ABCG2(9429)
詳細
SOLVO Biotechnology offers ready-to-use assay kits for efflux and uptake transporters. SOLVO′s PREDEASY™ ATPase Assay Kit provides everything necessary to conduct ATPase activation and inhibition assays to better understand the nature of the interaction between a compound and the transporter. By measuring ATPase activity, both activation and inhibition of transporters can be investigated.
アプリケーション
SOLVO′s PREDEASY™ ATPase Assay Kit is a unique product for measuring the ATPase-dependent drug - ABC membrane transporter interaction in a convenient, "ready-to-assay" package. The ATPase assay is an in vitro membrane assay designed to indicate the nature of the interaction between the compound and the transporter. By measuring ATPase activity, both activation and inhibition of transporters can be investigated using membranes from baculovirus-infected insect cells or mammalian cell membranes containing high levels of human or rodent wild-type transporters. ABC transporters mediate the transport of substrates against a concentration gradient using energy derived from ATP hydrolysis, which is proportional to the transporter activity and could easily be detected with a colorimetric method.
PREDIVEZ™ PREDEASY™ Kits contain all reagents (chemicals) and solutions necessary to conduct ATPase activation and inhibition assays. You only have to add water. Each kit comes with a CD that includes the assay protocol, data sheet, SDS, electronic data processing, evaluation templates and the reference data. The recommended layout allows activation and inhibition measurements in duplicates for 8 concentrations of the test article on one 96-well plate with all of the relevant controls.
PREDEASY™ ATPase Assay Kits are available for 3 and 10 plates for the transporters recommended by the FDA and the EMA.
PREDIVEZ™ PREDEASY™ Kits contain all reagents (chemicals) and solutions necessary to conduct ATPase activation and inhibition assays. You only have to add water. Each kit comes with a CD that includes the assay protocol, data sheet, SDS, electronic data processing, evaluation templates and the reference data. The recommended layout allows activation and inhibition measurements in duplicates for 8 concentrations of the test article on one 96-well plate with all of the relevant controls.
PREDEASY™ ATPase Assay Kits are available for 3 and 10 plates for the transporters recommended by the FDA and the EMA.
物理的形状
Supplied as frozen membrane vesicles, containing 5 mg/ml membrane protein, labeled with volume, catalog number (transporter), date of production, protocols and necessary reagents sufficient for the analysis of 3 or 10, 96-well plates.
法的情報
SOLVO Biotechnology, Inc.より提供。
PREDEASY is a trademark of Solvo Biotechnology
PREDIVEZ is a trademark of Solvo Biotechnology
シグナルワード
Danger
危険有害性の分類
Acute Tox. 3 Oral - Aquatic Chronic 3 - Eye Irrit. 2 - Flam. Liq. 3 - Met. Corr. 1 - Skin Irrit. 2 - STOT SE 3
ターゲットの組織
Respiratory system
保管分類コード
3 - Flammable liquids
引火点(°F)
131.0 °F
引火点(℃)
55 °C
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
毒物及び劇物取締法
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PRTR
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消防法
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労働安全衛生法名称等を表示すべき危険物及び有害物
キットコンポーネントの情報を参照してください
労働安全衛生法名称等を通知すべき危険物及び有害物
キットコンポーネントの情報を参照してください
カルタヘナ法
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Jan Code
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最新バージョンのいずれかを選択してください：
Albert Mennone et al.
Drug metabolism and disposition: the biological fate of chemicals, 38(10), 1673-1678 (2010-07-06)
Breast cancer resistance protein (Bcrp) is a member of the ATP-binding cassette membrane transporter family, which is expressed apically in liver, kidney, and intestine epithelium. Recent reports suggest that in addition to xenobiotics, porphyrins, and food toxins, Bcrp can also
Márton Jani et al.
Biological & pharmaceutical bulletin, 32(3), 497-499 (2009-03-03)
The pharmacokinetics of sulfasalazine, an anti-inflammatory drug is influenced by ATP-binding cassette G2 (ABCG2) (breast cancer resistance protein (BCRP), mitoxantrone resistance protein (MXR)) both in vitro and clinically. Due to its low passive permeability, the intracellular concentration of sulfasalazine is
M Maliepaard et al.
Cancer research, 61(8), 3458-3464 (2001-04-20)
High expression of the Breast Cancer Resistance Protein (BCRP) gene has been shown to be involved in resistance to chemotherapeutic drugs. Knowledge of the localization of BCRP protein in normal tissues may help unravel the normal function of this protein.
Hiroshi Kodaira et al.
The Journal of pharmacology and experimental therapeutics, 333(3), 788-796 (2010-03-23)
A synergistic effect of P-glycoprotein (P-gp)/Abcb1a and breast cancer resistance protein (Bcrp)/Abcg2 was reported to limit the brain penetration of their common substrates. This study investigated this based on pharmacokinetics using Mdr1a/1b(-/-), Bcrp(-/-), and Mdr1a/1b(-/-)/Bcrp(-/-) mice. Comparison of the brain-
Kumie Kage et al.
International journal of cancer, 97(5), 626-630 (2002-01-25)
Breast cancer resistance protein (BCRP) is a half-molecule ABC transporter highly expressed in mitoxantrone-resistant cells. In our study we established PA317 transfectants expressing Myc-tagged BCRP (MycBCRP) or HA-tagged BCRP (HABCRP). The exogenous BCRP protein migrated as a 70-kDa protein in
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