SRP0239

EGFR Active human

recombinant, expressed in baculovirus infected insect cells, ≥70% (SDS-PAGE)

• 別名: ERBB1, HER1, PIG61
• UNSPSCコード: 12352200
• NACRES: NA.32

特性

• 由来生物: human
• リコンビナント: expressed in baculovirus infected insect cells
• アッセイ: ≥70% (SDS-PAGE)
• 形状: aqueous solution
• 有効性: ≥0.14 units/mg
• 分子量: 87.3 kDa
• 包装: pkg of 10 μg
• 濃度: >0.02 mg/mL
• NCBIアクセッション番号: NM_005228
• UniProtアクセッション番号: P00533
• 輸送温度: dry ice
• 保管温度: −70°C
• 遺伝子情報: human ... EGFR(1956)

詳細

詳細

Epidermal growth factor receptor (EGFR) is a transmembrane protein and a member of a family of receptor tyrosine kinases (RTK) called avian erythroblastosis oncogene (BErbB). It contains a ligand-binding domain in its extracellular region, one transmembrane domain, and a tyrosine kinase-containing domain in its cytosolic region. EGFR is encoded by the gene mapped to human chromosome 7p11.2.

アプリケーション

EGFR Active human has been used in:

• photo-cross-linking assays
• StavroX cross-linking site identification
• in vitro kinase assay

生物化学的／生理学的作用

Epidermal growth factor receptor (EGFR) functions as a receptor for EGF-family of proteins, which are peptide growth factors. EGFR and ligand play a crucial role early embryonic development and in the renewal of stem cells in tissues such as the gut, liver and skin. This receptor-ligand system is also involved in the pathogenesis and progression of various types of carcinomas. In cancer, EGFR pathways promotes cellular proliferation, angiogenesis, metastasis and apoptosis. The microRNA miR-218-5p interacts with and controls EGFR, and in non-small cell lung cancer (NSCLC), the levels of EGFR are inversely related to miR-218-5p. miR-218-5p inhibits proliferation and migration of lung cancer cells by binding with EGFR, and thus, has potential in future NSCLC therapies.

単位の定義

1ユニットは、pH 7.4、30°Cで1分あたり1 pmolのチロシン基質をリン酸化する酵素量と定義されています。

物理的形状

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol, 10 mM glutathione and 3 mM DTT.

調製ノート

氷上で解凍してください。最初に解凍した際、チューブを軽く遠心して内容物を完全に回収してください。酵素液は一回の使用量ずつ分注してください。分注した酵素液は希釈せずそのまま-70°Cで保存してください。注:酵素は凍結融解の繰り返しに対して非常に不安定です。

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable