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B9754

Sigma-Aldrich

BIS-TRIS

≥98.0% (titration)

Synonym(s):

2,2-Bis(hydroxymethyl)-2,2′,2″-nitrilotriethanol, 2-Bis(2-hydroxyethyl)amino-2-(hydroxymethyl)-1,3-propanediol, Bis(2-hydroxyethyl)amino-tris(hydroxymethyl)methane

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About This Item

Empirical Formula (Hill Notation):
C8H19NO5
CAS Number:
6976-37-0
Molecular Weight:
209.24
Beilstein:
2205275
EC Number:
230-237-7
MDL number:
MFCD00002853
UNSPSC Code:
12161700
PubChem Substance ID:
24892155
NACRES:
NA.25

Assay

≥98.0% (titration)

form

crystalline powder

storage condition

dry at room temperature

technique(s)

UV/Vis spectroscopy: suitable
electrophoresis: suitable

color

white

useful pH range

5.8-7.2

pKa (25 °C)

6.5

solubility

H2O: 500 mg/mL, clear, colorless to very faintly yellow

suitability

suitable for Western blot
suitable for column chromatography of proteins

application(s)

diagnostic assay manufacturing
general analytical
microbiology

SMILES string

OCCN(CCO)C(CO)(CO)CO

InChI

1S/C8H19NO5/c10-3-1-9(2-4-11)8(5-12,6-13)7-14/h10-14H,1-7H2

InChI key

OWMVSZAMULFTJU-UHFFFAOYSA-N

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General description

Bis-tris methane, also known as BIS-TRIS or BTM, is a zwitterionic buffering agent widely employed in biochemical and biological research. It functions effectively in aqueous solutions within a pH range of 5.8 to 7.2 and demonstrates minimal shifts in its dissociation constant. Further, it can be used as a substitute for the highly toxic buffer cacodylate and is also recognized for its ability to form complexes with different metals.

Application

Bis-Tris has been used :
  • as a buffer for Electrophoresis
  • as a component of transfer buffer for Western Blotting

Features and Benefits

  • Suitable for Biological and Biochemical Research
  • Effective Buffering from pH 5.8-7.2 (25 °C) with a pKa of 6.5 (25 °C)

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H319

Hazard Classifications

Eye Irrit. 2

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Raman Kumar et al.
STAR protocols, 3(4), 101693-101693 (2022-09-20)
Immunoprecipitation (IP) of endogenously expressed proteins is one of the most biologically relevant techniques to identify protein-protein interactions. We describe an adaptable IP protocol reliant on a specific antibody to the target protein. We detail a quantitative proteomics workflow for
Lesley Cheng et al.
Kidney international, 86(2), 433-444 (2013-12-20)
Micro RNAs (miRNAs) have been shown to circulate in biological fluids and are enclosed in vesicles such as exosomes; they are present in urine and represent a noninvasive methodology to detect biomarkers for diagnostic testing. The low abundance of RNA
Elizabeth M MacDonald et al.
Disease models & mechanisms, 7(4), 471-481 (2014-02-08)
The purpose of our study was to compare two acquired muscle atrophies and the use of myostatin inhibition for their treatment. Myostatin naturally inhibits skeletal muscle growth by binding to ActRIIB, a receptor on the cell surface of myofibers. Because
Alastair W Skeffington et al.
Plant physiology, 165(2), 866-879 (2014-05-02)
The first step on the pathway of starch degradation in Arabidopsis (Arabidopsis thaliana) leaves at night is the phosphorylation of starch polymers, catalyzed by glucan, water dikinase (GWD). It has been suggested that GWD is important for the control of
Zahra Sabouri et al.
Proceedings of the National Academy of Sciences of the United States of America, 111(25), E2567-E2575 (2014-05-14)
The best-understood mechanisms for achieving antibody self/non-self discrimination discard self-reactive antibodies before they can be tested for binding microbial antigens, potentially creating holes in the repertoire. Here we provide evidence for a complementary mechanism: retaining autoantibodies in the repertoire displayed
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Protocols

Enzymatic Activity of Glucose-6-Phosphatase [EC 3.1.3.9]

To measure glucose-6-phosphatase activity, the Taussky-Shorr method is used. This method is a spectrophotometric stop-rate determination assay that is measured at 660 nm.

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