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B6755

Sigma-Aldrich

BIS-TRIS propane

≥99.0% (titration)

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Synonym(s):
1,3-Bis[tris(hydroxymethyl)methylamino]propane, 2,2′-(Propane-1,3-diylbis(azanediyl))bis(2-(hydroxymethyl)propane-1,3-diol)
Linear Formula:
CH2[CH2NHC(CH2OH)3]2
CAS Number:
Molecular Weight:
282.33
Beilstein:
1786109
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.25

Quality Level

Assay

≥99.0% (titration)

form

powder

useful pH range

6.3-9.5

pKa (25 °C)

(1) 6.8, (2) 9.0

mp

164-165 °C (lit.)

solubility

water: 30 % (w/w), clear, colorless

application(s)

diagnostic assay manufacturing

storage temp.

room temp

SMILES string

OCC(CO)(CO)NCCCNC(CO)(CO)CO

InChI

1S/C11H26N2O6/c14-4-10(5-15,6-16)12-2-1-3-13-11(7-17,8-18)9-19/h12-19H,1-9H2

InChI key

HHKZCCWKTZRCCL-UHFFFAOYSA-N

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1 of 4

This Item
B9410PHG0005RDD014
BIS-TRIS propane ≥99.0% (titration)

Sigma-Aldrich

B6755

BIS-TRIS propane

-
BIS-TRIS propane BioXtra, ≥99.0% (titration)

Sigma-Aldrich

B9410

BIS-TRIS propane

Premium Grade
BIS-TRIS propane

SAFC

PHG0005

BIS-TRIS propane

-
BIS-TRIS propane anhydrous, free-flowing, Redi-Dri™, ≥99.0%

Sigma-Aldrich

RDD014

BIS-TRIS propane

-
form

powder

form

powder

form

powder

form

powder

mp

164-165 °C (lit.)

mp

164-165 °C (lit.)

mp

164-165 °C (lit.)

mp

164-165 °C (lit.)

solubility

water: 30 % (w/w), clear, colorless

solubility

H2O: 1 M, clear, colorless

solubility

-

solubility

H2O: 30 % (w/w), clear, colorless

application(s)

diagnostic assay manufacturing

application(s)

diagnostic assay manufacturing

application(s)

-

application(s)

-

storage temp.

room temp

storage temp.

room temp

storage temp.

-

storage temp.

-

General description

Bis-Tris Propane buffer, also known as BTP, is a zwitterionic biochemical buffer utilized for pH regulation in various laboratory applications, particularly in molecular biology, biochemistry, and electrophoresis. Its wide buffering range, spanning from pH 6.3 to 9.5, is attributed to its two pKa values of 6.8 (pKa1) and 9.0 (pKa2), which are close. This wide range makes it highly versatile for maintaining stable pH conditions in a variety of experiments.

Furthermore, Bis-Tris Propane is a water-soluble buffer substance that plays a crucial role in enhancing the stability and activity of restriction enzymes. Specifically, its buffering capacity is advantageous for experiments that require pH levels as low as 6-7. When compared to Tris buffer, which has a poor buffering capacity below pH 7.5 and exhibits significant pKa fluctuations with temperature changes, Bis-Tris Propane proves to be a superior choice. Additionally, it finds application in conjunction with hydrochloric acid buffer to stabilize farnesyl diphosphate, particularly when isolated from Saccharomyces cerevisiae strains. Notably, it can also serve as a ligand, forming dinuclear hydroxo complexes with Lanthanides(III).

Application

BIS-TRIS Propane may be used in the following studies:
  • as a buffer to investigate the influence of anions with varying nucleophilic properties on the autoxidation of oxymyoglobin (MbO2).
  • for purification of glucose-binding protein from membranes of Sulfolobus solfataricus.
  • as a buffer during GeO2 mineralisation.
  • as a buffer component in a study pertaining to environmental research
  • as a buffer component to maintain optimal pH conditions during the purification and preparation of engineered enzymes

Features and Benefits

  • Suitable for Biological and Biochemical Research
  • Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications
  • Effective Buffering from pH 6.3-9.5 (25 °C) with a pKa of 6.8 and 9.0 (25 °C)
  • Highly soluble in water

Other Notes

Easily compare specifications for BIS-TRIS propane products with the BIS-TRIS propane specification table.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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CAPS

S V Albers et al.
Journal of bacteriology, 181(14), 4285-4291 (1999-07-10)
The archaeon Sulfolobus solfataricus grows optimally at 80 degrees C and pH 2.5 to 3.5 on carbon sources such as yeast extracts, tryptone, and various sugars. Cells rapidly accumulate glucose. This transport activity involves a membrane-bound glucose-binding protein that interacts
Y Satoh et al.
The Journal of biological chemistry, 256(20), 10272-10275 (1981-10-25)
In the presence of a salt in an excess amount, the oxidation of native MbO2 was enhanced considerably above the normal autoxidation in buffr alone with the formation of the corresponding MetMb . anion complex. This anion-induced oxidation of MbO2
Engineering the Organophosphorus Acid Anhydrolase Enzyme for Increased Catalytic Efficiency and Broadened Stereospecificity on Russian VX
Daczkowski CM et al.
Biochemistry, 54, 6423-6433 (2015)
Estefania Boix et al.
Dalton transactions (Cambridge, England : 2003), 43(44), 16902-16910 (2014-10-11)
We demonstrate the use of silk based proteins to control the particle/crystallite size during GeO2 formation, using a bio-mimetic approach at circumneutral pH and ambient temperature. Multicrystalline GeO2 was prepared from germanium tetraethoxide (TEOG) in the presence of different silk-based
Elisabeth Dohmann Chang et al.
Environmental science & technology, 55(10), 6848-6856 (2021-03-17)
Water pH is predicted to affect the uptake of ionizable pharmaceuticals in fish. The current study used an in vitro primary fish gill cell culture system to assess the effect of pH values in the range of 4.5-8.75 on the

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