861049
Azure A chloride
certified by the Biological Stain Commission, Dye content 70 %
동의어(들):
Methylene blue
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모든 사진(3)
About This Item
실험식(Hill 표기법):
C14H14ClN3S
CAS Number:
Molecular Weight:
291.80
색상 지수 번호:
52005
Beilstein:
3922287
EC Number:
MDL number:
UNSPSC 코드:
12171500
PubChem Substance ID:
NACRES:
NA.47
추천 제품
Grade
certified by the Biological Stain Commission
Quality Level
양식
powder
구성
Dye content, 70%
색상
blue to very dark blue
very dark green
mp
290 °C (dec.) (lit.)
solubility
H2O: 1 mg/mL
λmax
633 nm
ε (흡광계수)
≥11000 at 242-246 nm in H2O at -1
≥26000 at 287-291 nm in H2O
≥34000 at 620-634 μm in H2O
응용 분야
diagnostic assay manufacturing
hematology
histology
저장 온도
room temp
SMILES string
NC1=CC(SC(C2=N3)=CC(C=C2)=[N+](C)C)=C3C=C1.[Cl-]
InChI
1S/C14H13N3S.ClH/c1-17(2)10-4-6-12-14(8-10)18-13-7-9(15)3-5-11(13)16-12;/h3-8,15H,1-2H3;1H
InChI key
NALREUIWICQLPS-UHFFFAOYSA-N
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애플리케이션
Azure A has been used to determine the amount of charged dextran sulfate in the nanoparticles for the study involving incorporation of heparin-binding proteins into preformed dextran sulfate-chitosan nanoparticles.
생화학적/생리학적 작용
The combination of azure A and silver staining is used for the quantification of glycosaminoglycans in agarose gels. It is also used for the staining of chromosomes in the nucleus. Azure A is a main component of the azure B/polychromed methylene blue-eosin stains family, also referred to as the Romanowsky stain. Azure A is a basic cationic dye.
적합성
Certified for use in Lillie′s modified Nocht′s method and the NCCLS method for blood smears.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
개인 보호 장비
Eyeshields, Gloves, type N95 (US)
이미 열람한 고객
How Romanowsky stains work and why they remain valuable - including a proposed universal Romanowsky staining mechanism and a rational troubleshooting scheme.
Horobin RW
Biotechnic & Histochemistry, 86, 36-51 (2011)
Heterochromatin in human male leukocytes.
Lima-de-Faria A and Reitalu J
The Journal of Cell Biology, 16.2, 315-322 (1963)
Quantification and characterization of glycosaminoglycans at the nanogram level by a combined azure A-silver staining in agarose gels.
van de Lest CH, et al.
Analytical Biochemistry, 221, 356-361 (1994)
Paula Zaman et al.
International journal of nanomedicine, 11, 6149-6159 (2016-12-07)
Incorporation of proteins into dextran sulfate (DS)-chitosan (CS) nanoparticles (DSCS NPs) is commonly performed using entrapment procedures, in which protein molecules are mixed with DS and CS until particle formation occurs. As DS is an analog of heparin, the authors
F Battaglini et al.
Bioconjugate chemistry, 5(5), 430-435 (1994-09-01)
The bimolecular rate constants for the reactions of five organic two-electron redox mediators with reduced glucose oxidase (GOx) were determined by measuring voltammetric electrocatalytic currents at glassy carbon electrodes in the presence of excess glucose under anaerobic conditions. The mediators
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