800800C

08:0 DG

1,2-dioctanoyl-sn-glycerol, chloroform solution

• 동의어(들): DG(8:0/8:0/0:0)
• 실험식(Hill 표기법): C₁₉H₃₆O₅
• CAS Number: 60514-48-9
• Molecular Weight: 344.49
• MDL number: MFCD00036835
• UNSPSC 코드: 12352211
• NACRES: NA.25

속성

• 분석: >99% (TLC)
• 양식: liquid
• 포장: pkg of 1 × 5 mL (800800C-10mg); pkg of 1 × 5 mL (800800C-25mg)
• 제조업체/상표: Avanti Research^™ - A Croda Brand 800800C
• 농도: 2 mg/mL (800800C-10mg); 5 mg/mL (800800C-25mg)
• 지질 유형: neutral lipids; neutral glycerides
• 배송 상태: dry ice
• 저장 온도: −20°C
• SMILES string: O([C@H](COC(=O)CCCCCCC)CO)C(=O)CCCCCCC
• InChI: 1S/C19H36O5/c1-3-5-7-9-11-13-18(21)23-16-17(15-20)24-19(22)14-12-10-8-6-4-2/h17,20H,3-16H2,1-2H3/t17-/m0/s1
• InChI key: ZQBULZYTDGUSSK-KRWDZBQOSA-N

설명

일반 설명

08:0 DG (1,2-dioctanoyl-sn-glycerol), also called as di-C8, is a cell-permeable and a short-chain diacyl glycerol.

In biochemical signaling, diacylglycerol (DAG) functions as a second messenger signaling lipid, and is a product of the hydrolysis of the phospholipid PIP2 (phosphatidylinositolbisphosphate) by the enzyme phospholipase C (PLC) (a membrane-bound enzyme) that, through the same reaction, produces inositol trisphosphate (IP3). Although inositol trisphosphate (IP3) diffuses into the cytosol, DAG remains within the plasma membrane due to its hydrophobic properties. IP3 stimulates the release of calcium ions from the smooth endoplasmic reticulum, whereas DAG is a physiological activator of protein kinase C (PKC). The production of DAG in the membrane facilitates translocation of PKC from the cytosol to the plasma membrane.
Diacylglycerol mimicks the effects of the tumor-promoting compounds phorbol esters.

애플리케이션

08:0 DG may be used as a component of phosphatidylinositol 3-phosphate (PI3P) liposomes for vacuolar morphogenesis protein 7 (Vam7-6A) binding studies. It has been used in T-cell lymphoma invasion and metastasis–inducing protein 1 (TIAM1) transfection studies.

생화학적/생리학적 작용

08:0 DG (1,2-dioctanoyl-sn-glycerol) binds to the epidermal growth factor (EGF) receptor similar to 4β-phorbol 12β-myristate 13α-acetate (PMA). It also elevates the phosphoserine and phosphothreonine levels. DAG DiC8 activates protein kinase C (PKC). DiC8 stimulates the release of superoxide in neutrophils. It also inhibits the nicotine and clothianidin induced currents in neurosecretory cells and downregulates nicotinic acetylcholine receptor α1 subunit 1 (nAChR1).

포장

30 mL Amber Narrow Mouth Glass Bottle with Screw Cap (800800C-10mg)

30 mL Amber Narrow Mouth Glass Bottle with Screw Cap (800800C-25mg)

저장 및 안정성

Diacylglycerols are conveniently stored in chloroform solutions in glass vials with PTFE-lined caps at -20°C. Under these conditions acyl migration is minimal. Avoid plastic when handling chloroform solutions.

기타 정보

Delivery to cells:
Dry samples of diacylglycerol in chloroform, using a stream of nitrogen. Dissolve the residue in an appropriate volume of ethanol or DMSO, then dilute to the desired aqueous medium.

Effective concentration:
Most biological responses saturate at 20 to 250 μM sn-1,2-dioctanoylglycerol. Only sn-1,2 isomers appear to be active.

Precaution: Since short chain Diacylglycerols mimic effects of the tumor-promoting phorbol diesters in a number of biological systems, extra care should be employed in their handling. Treatment of solutions, vessels and other articles with 1N NaOH before washing or discarding will destroy diacylglycerols.

법적 정보

Avanti Research is a trademark of Avanti Polar Lipids, LLC

안전성 정보

• 픽토그램: GHS06,GHS08
• 신호어: Danger
• 유해 및 위험 성명서: H302,H315,H319,H331,H336,H351,H361d,H372,H412
• 예방조치 성명서: P201 - P273 - P301 + P312 + P330 - P302 + P352 - P304 + P340 + P311 - P308 + P313
• Hazard Classifications: Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Carc. 2 - Eye Irrit. 2 - Repr. 2 - Skin Irrit. 2 - STOT RE 1 - STOT SE 3
• 표적 기관: Central nervous system, Liver,Kidney
• WGK: WGK 3